TY - JOUR
T1 - Dietary supplementation of fenugreek seed extract and choline-docosahexaenoic acid on oxidative stress and neurodegeneration in ovariectomized rats with bilateral common carotid artery occlusion
AU - Konuri, Anjaneyulu
AU - Bhat, Kumar M.R.
AU - Rai, Kiranmai S.
AU - Nagamma, T.
AU - Thangarajan, Rajesh
AU - Sivakumar, G.
N1 - Funding Information:
The authors thank Manipal Academy of Higher Education for providing research facilities.
Publisher Copyright:
© RJPT All right reserved.
PY - 2021/4
Y1 - 2021/4
N2 - As impairment of memory and ischemic brain injury is more common in female of menopausal age, the current study aims to evaluate the protective role of fenugreek seed extract and choline-Docosahexaenoic acid (DHA) on cognitive behavior, oxidative stress and neurodegeneration in ovariectomized-cerebral ischemic rats. Cerebral ischemia was induced by bilateral common carotid artery occlusion (BCCAO) surgery to ovariectomized adult female wistar rats. Fenugreek, choline-DHA and combination of fenugreek with choline-DHA was supplemented for 30days after ovariectomy and continued for 14 more days after BCCAO surgery. Effect of this supplementation on learning and memory, brain oxidative stress and neurodegeneration in the CA1, CA3 hippocampal sub-regions were analyzed. Ovariectomized-cerebral ischemic rats demonstrated learning and memory impairment when subjected to passive avoidance task. Further, these rats also had increased brain oxidative stress, and neurodegeneration in CA1, CA3 sub-regions of hippocampus. Conversely, combined supplementation of fenugreek with choline-DHA to ovariectomized-cerebral ischemic rats exhibited a significant improvement in their learning and memory abilities, with reduced oxidative stress and neurodegeneration in CA1 and CA3 sub-regions of hippocampus.
AB - As impairment of memory and ischemic brain injury is more common in female of menopausal age, the current study aims to evaluate the protective role of fenugreek seed extract and choline-Docosahexaenoic acid (DHA) on cognitive behavior, oxidative stress and neurodegeneration in ovariectomized-cerebral ischemic rats. Cerebral ischemia was induced by bilateral common carotid artery occlusion (BCCAO) surgery to ovariectomized adult female wistar rats. Fenugreek, choline-DHA and combination of fenugreek with choline-DHA was supplemented for 30days after ovariectomy and continued for 14 more days after BCCAO surgery. Effect of this supplementation on learning and memory, brain oxidative stress and neurodegeneration in the CA1, CA3 hippocampal sub-regions were analyzed. Ovariectomized-cerebral ischemic rats demonstrated learning and memory impairment when subjected to passive avoidance task. Further, these rats also had increased brain oxidative stress, and neurodegeneration in CA1, CA3 sub-regions of hippocampus. Conversely, combined supplementation of fenugreek with choline-DHA to ovariectomized-cerebral ischemic rats exhibited a significant improvement in their learning and memory abilities, with reduced oxidative stress and neurodegeneration in CA1 and CA3 sub-regions of hippocampus.
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U2 - 10.52711/0974-360X.2021.00381
DO - 10.52711/0974-360X.2021.00381
M3 - Article
AN - SCOPUS:85108649346
SN - 0974-3618
VL - 14
SP - 2153
EP - 2161
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 4
ER -