TY - JOUR
T1 - Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures
AU - Madhusoodanan, Geethu
AU - Hariharapura, Raghu Chandrashekar
AU - Somashekara, Divyashree
N1 - Funding Information:
This work was ostensibly supported by The Department of Biotechnology (DBT), Government of India, (PR 18430/BIC/101/703/2016) and grateful to The Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education (MAHE), India; for providing the facilities to carry out the research work.
Publisher Copyright:
© 2021, The Author(s).
PY - 2022/4
Y1 - 2022/4
N2 - Conventional biopolymers resembling synthetic polymers produced from microorganisms, polyhydroxyalkanoate (PHA) synthesized utilizing renewable resources have gained supreme attention recently. PHA accumulation within the microbial cell is an innate capability of bacteria to store carbon and energy when nutrient imbalance pertains. Gram positive Bacillus endophyticus capable of synthesizing PHA was focused in this study. Study focuses on the possibility of attaining high PHA yield in relation to the varying dissolved oxygen levels induced during production phase. There was a gradual increment in PHA production from 34.5 to 53.03% when cultivated in bioreactor that maintained least dissolved oxygen of 0.4 mg/L at 32 °C. The metabolic flux of organism was altered during oxygen stress brought by varying agitation rate and volume resulting in the accumulation of Nicotinamide Adenine Dinucleotide Hydrogen (NADH), which led to increase in the overall PHA production. PHA yield was found to be favored by decreasing the oxygen supply thereby inducing an oxygen stress environment. This report was the first one that was correlating the hypothesis that links PHA yield and oxygen stress condition during production phase. PHA produced was characterized by FTIR and 1HNMR spectra in which the presence of Polyhydroxybutyrate was confirmed. Graphical abstract: [Figure not available: see fulltext.]
AB - Conventional biopolymers resembling synthetic polymers produced from microorganisms, polyhydroxyalkanoate (PHA) synthesized utilizing renewable resources have gained supreme attention recently. PHA accumulation within the microbial cell is an innate capability of bacteria to store carbon and energy when nutrient imbalance pertains. Gram positive Bacillus endophyticus capable of synthesizing PHA was focused in this study. Study focuses on the possibility of attaining high PHA yield in relation to the varying dissolved oxygen levels induced during production phase. There was a gradual increment in PHA production from 34.5 to 53.03% when cultivated in bioreactor that maintained least dissolved oxygen of 0.4 mg/L at 32 °C. The metabolic flux of organism was altered during oxygen stress brought by varying agitation rate and volume resulting in the accumulation of Nicotinamide Adenine Dinucleotide Hydrogen (NADH), which led to increase in the overall PHA production. PHA yield was found to be favored by decreasing the oxygen supply thereby inducing an oxygen stress environment. This report was the first one that was correlating the hypothesis that links PHA yield and oxygen stress condition during production phase. PHA produced was characterized by FTIR and 1HNMR spectra in which the presence of Polyhydroxybutyrate was confirmed. Graphical abstract: [Figure not available: see fulltext.]
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U2 - 10.1007/s10668-021-01626-3
DO - 10.1007/s10668-021-01626-3
M3 - Article
AN - SCOPUS:85111385169
SN - 1387-585X
VL - 24
SP - 4641
EP - 4658
JO - Environment, Development and Sustainability
JF - Environment, Development and Sustainability
IS - 4
ER -
