TY - JOUR
T1 - Dynamic in vitro culture of bovine and human ovarian tissue enhances follicle progression and health
AU - Barbato, Vincenza
AU - Genovese, Vincenzo
AU - De Gregorio, Vincenza
AU - Di Nardo, Maddalena
AU - Travaglione, Angela
AU - De Napoli, Luigi
AU - Fragomeni, Gionata
AU - Zanetti, Elisabetta M.
AU - Adiga, Satish K.
AU - Mondrone, Giuseppe
AU - D’Hooghe, Thomas
AU - Zheng, Wengijng
AU - Longobardi, Salvatore
AU - Catapano, Gerardo
AU - Gualtieri, Roberto
AU - Talevi, Riccardo
N1 - Funding Information:
The study was supported by IVF-Research, Education, Development S.r.l., Via San Josemaria Escrivà 68, 81100 Caserta, Italy, and Merck Healthcare KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany, within the Medical Innovation Program (MIP) agreement. The contribution of Mr. Giuseppe Serratore and Mr. Giuseppe Morrone, Department of Mechanical, Energy and Management Engineering, University of Calabria, to the numerical simulations and to plots preparation is gratefully acknowledged.
Funding Information:
The study was supported by IVF-Research, Education, Development S.r.l., Via San Josemaria Escrivà 68, 81100 Caserta, Italy, and Merck Healthcare KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany, within the Medical Innovation Program (MIP) agreement. The contribution of Mr. Giuseppe Serratore and Mr. Giuseppe Morrone, Department of Mechanical, Energy and Management Engineering, University of Calabria, to the numerical simulations and to plots preparation is gratefully acknowledged.
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12
Y1 - 2023/12
N2 - In vitro ovarian cortical tissue culture, followed by culture of isolated secondary follicles, is a promising future option for production of mature oocytes. Although efforts have been made to improve the culture outcome by changing the medium composition, so far, most studies used static culture systems. Here we describe the outcome of 7 days cultures of bovine and human ovarian cortical tissue in a dynamic system using a novel perifusion bioreactor in comparison to static culture in conventional and/or gas permeable dishes. Findings show that dynamic culture significantly improves follicle quality and viability, percentage and health of secondary follicles, overall tissue health, and steroid secretion in both species. Model predictions suggest that such amelioration can be mediated by an enhanced oxygen availability and/or by fluid-mechanical shear stresses and solid compressive strains exerted on the tissue.
AB - In vitro ovarian cortical tissue culture, followed by culture of isolated secondary follicles, is a promising future option for production of mature oocytes. Although efforts have been made to improve the culture outcome by changing the medium composition, so far, most studies used static culture systems. Here we describe the outcome of 7 days cultures of bovine and human ovarian cortical tissue in a dynamic system using a novel perifusion bioreactor in comparison to static culture in conventional and/or gas permeable dishes. Findings show that dynamic culture significantly improves follicle quality and viability, percentage and health of secondary follicles, overall tissue health, and steroid secretion in both species. Model predictions suggest that such amelioration can be mediated by an enhanced oxygen availability and/or by fluid-mechanical shear stresses and solid compressive strains exerted on the tissue.
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U2 - 10.1038/s41598-023-37086-0
DO - 10.1038/s41598-023-37086-0
M3 - Article
AN - SCOPUS:85165354843
SN - 2045-2322
VL - 13
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 11773
ER -
