TY - JOUR
T1 - Effect of Amalaki rasayana on DNA damage and repair in randomized aged human individuals
AU - Vishwanatha, Udupi
AU - Guruprasad, Kanive P.
AU - Gopinath, Puthiya M.
AU - Acharya, Raviraj V.
AU - Prasanna, Bokkasa V.
AU - Nayak, Jayakrishna
AU - Ganesh, Rajeshwari
AU - Rao, Jayalaxmi
AU - Shree, Rashmi
AU - Anchan, Suchitra
AU - Raghu, Kothanahalli S.
AU - Joshi, Manjunath B.
AU - Paladhi, Puspendu
AU - Varier, Panniampilly M.
AU - Muraleedharan, Kollath
AU - Muraleedharan, Thrikovil S.
AU - Satyamoorthy, Kapaettu
PY - 2016/9/15
Y1 - 2016/9/15
N2 - Ethnopharmacological relevance Preparations from Phyllanthus emblica called Amalaki rasayana is used in the Indian traditional medicinal system of Ayurveda for healthy living in elderly. The biological effects and its mechanisms are not fully understood. Since the diminishing DNA repair is the hallmark of ageing, we tested the influence of Amalaki rasayana on recognized DNA repair activities in healthy aged individuals. Methods Amalaki rasayana was prepared fresh and healthy aged randomized human volunteers were administrated with either rasayana or placebo for 45 days strictly as per the traditional text. The DNA repair was analyzed in peripheral blood mononuclear cells before and after rasayana administration and after 45 days post-rasayana treatment regimen. UVC-induced DNA strand break repair (DSBR) based on extent of DNA unwinding by fluorometric analysis, nucleotide excision repair (NER) by flow cytometry and constitutive base excision repair (BER) by gap filling method were analyzed. Results Amalaki rasayana administration stably maintained/enhanced the DSBR in aged individuals. There were no adverse side effects. Further, subjects with different body mass index showed differential DNA strand break repair capacity. No change in unscheduled DNA synthesis during NER and BER was observed between the groups. Conclusion Intake of Amalaki rasayana by aged individuals showed stable maintenance of DNA strand break repair without toxic effects. However, there was no change in nucleotide and base excision repair activities. Results warrant further studies on the effects of Amalaki rasayana on DSBR activities.
AB - Ethnopharmacological relevance Preparations from Phyllanthus emblica called Amalaki rasayana is used in the Indian traditional medicinal system of Ayurveda for healthy living in elderly. The biological effects and its mechanisms are not fully understood. Since the diminishing DNA repair is the hallmark of ageing, we tested the influence of Amalaki rasayana on recognized DNA repair activities in healthy aged individuals. Methods Amalaki rasayana was prepared fresh and healthy aged randomized human volunteers were administrated with either rasayana or placebo for 45 days strictly as per the traditional text. The DNA repair was analyzed in peripheral blood mononuclear cells before and after rasayana administration and after 45 days post-rasayana treatment regimen. UVC-induced DNA strand break repair (DSBR) based on extent of DNA unwinding by fluorometric analysis, nucleotide excision repair (NER) by flow cytometry and constitutive base excision repair (BER) by gap filling method were analyzed. Results Amalaki rasayana administration stably maintained/enhanced the DSBR in aged individuals. There were no adverse side effects. Further, subjects with different body mass index showed differential DNA strand break repair capacity. No change in unscheduled DNA synthesis during NER and BER was observed between the groups. Conclusion Intake of Amalaki rasayana by aged individuals showed stable maintenance of DNA strand break repair without toxic effects. However, there was no change in nucleotide and base excision repair activities. Results warrant further studies on the effects of Amalaki rasayana on DSBR activities.
UR - http://www.scopus.com/inward/record.url?scp=84978253989&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978253989&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2016.06.062
DO - 10.1016/j.jep.2016.06.062
M3 - Article
C2 - 27364038
AN - SCOPUS:84978253989
SN - 0378-8741
VL - 191
SP - 387
EP - 397
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
ER -
