TY - JOUR
T1 - Effect of cholesterol on size distribution of freeze-thaw extruded liposomes
AU - Agarwal, S.
AU - Subramanian, G.
AU - Shetiya, P.
AU - Udupa, N.
N1 - Export Date: 10 November 2017
CODEN: IJSID
Correspondence Address: Udupa, N.; College of Pharmaceutical Sciences, MAHE, Manipal-576119, India; email: [email protected]
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PY - 2004
Y1 - 2004
N2 - The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freeze-thawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196° followed by 3 min thawing at 50° resulted in an anomalously large particle size for egg phosphatidylcholine/cholesterol formulations. When egg phosphatidylcholine/cholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.
AB - The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freeze-thawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196° followed by 3 min thawing at 50° resulted in an anomalously large particle size for egg phosphatidylcholine/cholesterol formulations. When egg phosphatidylcholine/cholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.
UR - https://www.scopus.com/pages/publications/77952834790
UR - https://www.scopus.com/pages/publications/77952834790#tab=citedBy
M3 - Article
AN - SCOPUS:77952834790
SN - 0250-474X
VL - 66
SP - 450
EP - 452
JO - Indian Journal of Pharmaceutical Sciences
JF - Indian Journal of Pharmaceutical Sciences
IS - 4
ER -