Effect of ethanolic extract of Cyanotis cristata leaves applied topically on wound healing in wistar rats

Anurag Pathak, Smita Shenoy, Sushil Kiran, Avinash Arivazahan, Deepak Nayak, Naveen Kumar, Amod Tilak, Manjunath Shetty

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Objective: The objective of this study was to assess the effect of ethanolic extract of Cyanotis cristata leaves when applied topically on healing of wound in Wistar rats. Methods: C. cristata leaves were evaluated for its effect on healing of wound in three models, namely, incision, excision, and burn wound. Each model included five groups, each consisting of 6 rats. The five groups were as follows: Group I rats (control and ointment base), Group II rats (standard and silver sulfadiazine), and Group III, Group IV, Group V (treated with C. cristata extract ointment of 0.5%, 1%, and 2%, respectively). Variables evaluated included breaking strength in incision wound while it was contraction rate and epithelialization in excision and burn wound. One-way analysis of variance and Tukey’s post hoc test was used to analyze data. Result: In incision wound, breaking strength in test group was significantly increased (p<0.001) as compared to control. In excision and burn wound of test group, time to epithelialization and contraction rate was significantly decreased (p<0.001). The granulation tissue from excision and burn wound showed increased collagen and less inflammatory cells in test groups in comparison to the control. Conclusion: The ethanolic extract of leaves of C. cristata, when applied topically, enhanced wound strength and accelerated healing of incision, excision, and burn wounds in rats.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalAsian Journal of Pharmaceutical and Clinical Research
Issue number12
Publication statusPublished - 01-01-2017

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)


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