Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus

M. S. Divyashree; T. R. Shamala

doi:10.1016/j.radphyschem.2008.08.010

Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus

M. S. Divyashree
, T. R. Shamala^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×10⁵ to 1.9×10⁵ after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×10⁵ to 2.3×10 ⁵ and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and ¹H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.

Original language	English
Pages (from-to)	147-152
Number of pages	6
Journal	Radiation Physics and Chemistry
Volume	78
Issue number	2
DOIs	https://doi.org/10.1016/j.radphyschem.2008.08.010
Publication status	Published - 01-02-2009

All Science Journal Classification (ASJC) codes

Radiation

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10.1016/j.radphyschem.2008.08.010

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title = "Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus",

abstract = "Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45\% and 54\% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×105 to 1.9×105 after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×105 to 2.3×10 5 and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and 1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.",

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doi = "10.1016/j.radphyschem.2008.08.010",

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T1 - Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus

AU - Divyashree, M. S.

AU - Shamala, T. R.

PY - 2009/2/1

Y1 - 2009/2/1

N2 - Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×105 to 1.9×105 after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×105 to 2.3×10 5 and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and 1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.

AB - Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×105 to 1.9×105 after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×105 to 2.3×10 5 and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and 1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.

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U2 - 10.1016/j.radphyschem.2008.08.010

DO - 10.1016/j.radphyschem.2008.08.010

M3 - Article

AN - SCOPUS:56349148315

SN - 0969-806X

VL - 78

SP - 147

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JO - Radiation Physics and Chemistry

JF - Radiation Physics and Chemistry

IS - 2

ER -

Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus

Abstract

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