Effect of laser dose and treatment schedule on excision wound healing in diabetic mice

The present study was undertaken to evaluate a He-Ne laser (632.8 nm; 7 mW; 4.02 mW cm ^-2; 15 mm spot size) dose and the treatment schedule on diabetic wound healing in a mouse model. Circular wounds of 15 mm diameter were created on streptozotocin induced diabetic Swiss albino mice, and were uniformly illuminated with the single exposure of various He-Ne laser doses of 1, 2, 3, 4 and 5 J cm ^-2 respectively. Further, the treatment schedule was also optimized by exposing the wounds with 3 J cm ^-2 at 0, 24 h, 48 h postwounding. Contraction kinetics, mean area under the curve and the mean healing time of the wounds were computed along with the collagen and the glucosamine levels in the wound ground tissues at various postwounding treatment schedules. Results of this study indicated that the single exposure of 3 J cm ^-2 laser dose applied immediately after the wounding caused a significant reduction in the mean area under the curve and the mean healing time along with the elevated levels of collagen and glucosamine contents in the tissue compared to the controls. In conclusion, He-Ne laser dose of 3 J cm ^-2 applied immediately after the wounding has demonstrated optimum wound healing compared to the other doses and treatment schedules. The present study was undertaken to evaluate a He-Ne laser dose and the treatment schedule on diabetic wound healing in Swiss albino mice. Circular wounds of 15 mm diameter were uniformly illuminated with the single exposure of various laser doses of 1, 2, 3, 4 and 5 J cm ^-2 respectively. An attempt was also made to optimize the treatment schedule by exposing the wounds with 3 J cm ^-2 at 0, 24 h and 48 h postwounding. Biophysical and biochemical measurements of the wound tissues exposed to 3 J cm ^-2 immediately after the wounding demonstrated significant photobiomodulatory effect on diabetic wound healing.