Effect of Nishamalaki on cerebellum and cerebral cortex in Rat Model of Diabetic Depression

Diabetes mellitus is a metabolic disorder. The incidence of depression is two to three times more in patients with diabetes when compared to healthy population. Depression is ignored in diabetic patients. The present research is focused on Sustainable Development Goal 3 (SDG 3) which targets "Good Health and Well-being". Nishamalaki is an Ayurvedic preparation which contains a mixture of amla (Emblica officinalis) and Turmeric (Curcuma longa). Till date, no studies has been explored on the antidepressant role of Nishamalaki in diabetic depression. Wistar rats were randomly divided into control groups and experimental groups. The experimental groups were injected with streptozotocin (40mg/kg Body weight), based on the 15 days chronic unpredictable stress exposure and treatment with Nishamalaki these rats were further subdivided into various subgroups. At the end of the 15th day, after the behavior and cognitive function analysis, the rats were anaesthetized, blood was collected through cardiac puncture for the estimation of serum cortisol. Cortex and Cerebellum was separated from the brain tissue for the biochemical analysis. The blood glucose level, serum cortisol was significantly decreased in the treated groups. Moreover, Lipid peroxidation level in the cerebellum and cortex significantly decreased(P<0.0001) and antioxidant level in these tissues significantly increased (P<0.0001). Sensory motor coordination and behavioral functions, cognitive functions showed significant improvement (P<0.0001) in the treated group. The level of Anxiety significantly decreased in the Nishamalaki treated group. The present study, proved the antidepressant role of Nishamalaki along with the stabilized action of hypoglycemia.