Effect of Wheatgrass Juice on Fertility Changes Induced by Cyclophosphamide in Male Wistar Albino Rats

Akshatha P. Kamath, Navin Patil, Amrita Parida, Karthik Rao, Nadeem G. Khan, Shanti Gurung, Afreen Begum H. Itagi, V. Manju, Heraa Islam, Aditi Kapoor, Mohsin Nazeer, Shreya Hegde, Nikhilesh Anand

Research output: Contribution to journalArticlepeer-review


In the present study, reproductive toxicity caused by cyclophosphamide on sperm parameters, biochemical parameters, histopathology and protective effect of wheatgrass juice was examined. There were total 6 groups of adult Wistar albino rats. Each group had 6 rats. After 24hrs of the last treatment, rats were sacrificed by cervical dislocation and sperm parameters like sperm count and motility, plasma malonaldehyde (MDA) levels, catalase (CAT) levels, superoxide dismutase (SOD) and glutathione (GSH) activities in testicular tissue, and testicular histopathological changes in the testicular tissue were examined. Administration of cyclophosphamide showed an increase in plasma MDA level and decrease in SOD, GSH and Catalase activity. These values were statistically significant. However, wheatgrass juice treatment along with cyclophosphamide markedly showed improvement in reducing cyclophosphamideinduced oxidative stress, and normalized sperm characteristics and testicular histopathology. Thus, in conclusion, cyclophosphamide causes fertility changes which leads to male infertility, whereas wheatgrass juice has a protective effect on reversing the testicular damage and male infertility.

Original languageEnglish
Pages (from-to)1011-1017
Number of pages7
JournalBiomedical and Pharmacology Journal
Issue number2
Publication statusPublished - 06-2023

All Science Journal Classification (ASJC) codes

  • Pharmacology


Dive into the research topics of 'Effect of Wheatgrass Juice on Fertility Changes Induced by Cyclophosphamide in Male Wistar Albino Rats'. Together they form a unique fingerprint.

Cite this