Effects of adriamycin and daunomycin on ornithine decarboxylase activity and DNA synthesis in mouse epidermal cells

K. Satyamoorthy; E. M. Perchellet; J. P. Perchellet

doi:10.1016/0887-2333(92)90053-T

Effects of adriamycin and daunomycin on ornithine decarboxylase activity and DNA synthesis in mouse epidermal cells

K. Satyamoorthy
, E. M. Perchellet
, J. P. Perchellet^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60%, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50% or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca²⁺-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.

Original language	English
Pages (from-to)	459-463
Number of pages	5
Journal	Toxicology in Vitro
Volume	6
Issue number	5
DOIs	https://doi.org/10.1016/0887-2333(92)90053-T
Publication status	Published - 1992

All Science Journal Classification (ASJC) codes

Toxicology

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@article{a94991f6a149446a9e95b05c8fb35b3a,

title = "Effects of adriamycin and daunomycin on ornithine decarboxylase activity and DNA synthesis in mouse epidermal cells",

abstract = "Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60\%, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50\% or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca2+-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.",

author = "K. Satyamoorthy and Perchellet, \{E. M.\} and Perchellet, \{J. P.\}",

year = "1992",

doi = "10.1016/0887-2333(92)90053-T",

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volume = "6",

pages = "459--463",

journal = "Toxicology in Vitro",

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TY - JOUR

T1 - Effects of adriamycin and daunomycin on ornithine decarboxylase activity and DNA synthesis in mouse epidermal cells

AU - Satyamoorthy, K.

AU - Perchellet, E. M.

AU - Perchellet, J. P.

PY - 1992

Y1 - 1992

N2 - Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60%, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50% or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca2+-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.

AB - Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60%, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50% or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca2+-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.

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DO - 10.1016/0887-2333(92)90053-T

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VL - 6

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JO - Toxicology in Vitro

JF - Toxicology in Vitro

IS - 5

ER -

Effects of adriamycin and daunomycin on ornithine decarboxylase activity and DNA synthesis in mouse epidermal cells

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