TY - JOUR
T1 - Elucidating Methods for Isolation and Quantification of Exosomes
T2 - A Review
AU - Kurian, Talitha Keren
AU - Banik, Soumyabrata
AU - Gopal, Dharshini
AU - Chakrabarti, Shweta
AU - Mazumder, Nirmal
N1 - Funding Information:
We received financial support from the Ministry of Science and Technology, Department of Science and Technology (DST), Government of India (Project Number-DST/INT/BLG/P-3/2019). We thank Dr. K. Satyamoorthy, Director, Manipal School of Life Sciences (MSLS), Manipal for his encouragement. Authors thank Dr. K. K. Mahato, Head of Department of Biophysics, MSLS for his constant support and Manipal Academy of Higher Education (MAHE), Manipal, India, for providing the infrastructure needed.
Publisher Copyright:
© 2021, The Author(s).
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/4
Y1 - 2021/4
N2 - Exosomes are the smallest extracellular vesicles present in most of the biological fluids. They are found to play an important role in cell signaling, immune response, tumor metastasis, etc. Studies have shown that these vesicles also have diagnostic and therapeutic roles for which their accurate detection and quantification is essential. Due to the complexity in size and structure of exosomes, even the gold standard methods face challenges. This comprehensive review discusses the various standard methods such as ultracentrifugation, ultrafiltration, size-exclusion chromatography, precipitation, immunoaffinity, and microfluidic technologies for the isolation of exosomes. The principle of isolation of each method is described, as well as their specific advantages and disadvantages. Quantification of exosomes by nanoparticle tracking analysis, flow cytometry, tunable resistive pulse sensing, electron microscopy, dynamic light scattering, and microfluidic devices are also described, along with the applications of exosomes in various biomedical domains.
AB - Exosomes are the smallest extracellular vesicles present in most of the biological fluids. They are found to play an important role in cell signaling, immune response, tumor metastasis, etc. Studies have shown that these vesicles also have diagnostic and therapeutic roles for which their accurate detection and quantification is essential. Due to the complexity in size and structure of exosomes, even the gold standard methods face challenges. This comprehensive review discusses the various standard methods such as ultracentrifugation, ultrafiltration, size-exclusion chromatography, precipitation, immunoaffinity, and microfluidic technologies for the isolation of exosomes. The principle of isolation of each method is described, as well as their specific advantages and disadvantages. Quantification of exosomes by nanoparticle tracking analysis, flow cytometry, tunable resistive pulse sensing, electron microscopy, dynamic light scattering, and microfluidic devices are also described, along with the applications of exosomes in various biomedical domains.
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U2 - 10.1007/s12033-021-00300-3
DO - 10.1007/s12033-021-00300-3
M3 - Review article
AN - SCOPUS:85099759118
SN - 1073-6085
VL - 63
SP - 249
EP - 266
JO - Molecular Biotechnology
JF - Molecular Biotechnology
IS - 4
ER -