TY - JOUR
T1 - Emerging Pathogens in Planetary Health and Lessons from Comparative Genome Analyses of Three Clostridia Species
AU - Tanwar, Ankit Singh
AU - Shruptha, Padival
AU - Jnana, Apoorva
AU - Brand, Angela
AU - Ballal, Mamatha
AU - Satyamoorthy, Kapaettu
AU - Murali, Thokur Sreepathy
N1 - Funding Information:
The authors thank Manipal Academy of Higher Education, Technology Information Forecasting Assessment Council—Centre of Relevance and Excellence in Pharmacogenomics (TIFAC-CORE), and DBT BUILDER—Interdisciplinary Life Science Programme for Advance Research and Education (DB-ILSPARE) for the support. A.S.T. and A.J. thank MAHE for Dr. TMA Pai PhD Scholarship and P.S. thanks Lady TATA Memorial Trust for Junior Research Fellowship.
Publisher Copyright:
© Copyright 2023, Mary Ann Liebert, Inc., publishers 2023.
PY - 2023/6/1
Y1 - 2023/6/1
N2 - Clostridioides difficile (CD) is a major planetary health burden. A Gram-positive opportunistic pathogen, CD, colonizes the large intestine and is implicated in sepsis, pseudomembranous colitis, and colorectal cancer. C. difficile infection typically following antibiotic exposure results in dysbiosis of the gut microbiome, and is one of the leading causes of diarrhea in the elderly population. While several studies have focused on the toxigenic strains of CD, gut commensals such as Clostridium butyricum (CB) and Clostridium tertium (CT) could harbor toxin/virulence genes, and thus pose a threat to human health. In this study, we sequenced and characterized three isolates, namely, CT (MALS001), CB (MALS002), and CD (MALS003) for their antimicrobial, cytotoxic, antiproliferative, genomic, and proteomic profiles. Although in vitro cytotoxic and antiproliferative potential were observed predominantly in CD MALS003, genome analysis revealed pathogenic potential of CB MALS002 and CT MALS001. Pangenome analysis revealed the presence of several accessory genes typically involved in fitness, virulence, and resistance characteristics in the core genomes of sequenced strains. The presence of an array of virulence and antimicrobial resistance genes in CB MALS002 and CT MALS001 suggests their potential role as emerging pathogens with significant impact on planetary health.
AB - Clostridioides difficile (CD) is a major planetary health burden. A Gram-positive opportunistic pathogen, CD, colonizes the large intestine and is implicated in sepsis, pseudomembranous colitis, and colorectal cancer. C. difficile infection typically following antibiotic exposure results in dysbiosis of the gut microbiome, and is one of the leading causes of diarrhea in the elderly population. While several studies have focused on the toxigenic strains of CD, gut commensals such as Clostridium butyricum (CB) and Clostridium tertium (CT) could harbor toxin/virulence genes, and thus pose a threat to human health. In this study, we sequenced and characterized three isolates, namely, CT (MALS001), CB (MALS002), and CD (MALS003) for their antimicrobial, cytotoxic, antiproliferative, genomic, and proteomic profiles. Although in vitro cytotoxic and antiproliferative potential were observed predominantly in CD MALS003, genome analysis revealed pathogenic potential of CB MALS002 and CT MALS001. Pangenome analysis revealed the presence of several accessory genes typically involved in fitness, virulence, and resistance characteristics in the core genomes of sequenced strains. The presence of an array of virulence and antimicrobial resistance genes in CB MALS002 and CT MALS001 suggests their potential role as emerging pathogens with significant impact on planetary health.
UR - https://www.scopus.com/pages/publications/85162253986
UR - https://www.scopus.com/pages/publications/85162253986#tab=citedBy
U2 - 10.1089/omi.2023.0034
DO - 10.1089/omi.2023.0034
M3 - Article
C2 - 37195730
AN - SCOPUS:85162253986
SN - 1536-2310
VL - 27
SP - 247
EP - 259
JO - OMICS A Journal of Integrative Biology
JF - OMICS A Journal of Integrative Biology
IS - 6
ER -
