TY - JOUR
T1 - Establishment of Tissue Biodistribution and Blood Clearance Rates of Intravenously Administered Radioactive 51Cr3+ in New Zealand White Rabbits
AU - Baby, Prathap Moothamadathil
AU - Kumar, Pramod
AU - Kumar, Rajesh
AU - Jacob, Sanu Susan
AU - Rawat, Dinesh
AU - Nair, Binu V.Sreekumaran
AU - Karun, Kalesh M.
N1 - Funding Information:
We acknowledge the support extended by the department of nuclear medicine, Kasturba Medical College - Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India and Central Animal Research Facility, Manipal Academy of Higher Education, Manipal, Karnataka, India. We also thank Dr. Prakash P Y., Associate Professor, Department of Microbiology, Kasturba Medical College – Manipal, Manipal Academy of Higher Education for the guidance and support in drafting this manuscript
Funding Information:
Funding: “This research received no external funding” Acknowledgments: We acknowledge the support extended by the department of nuclear medicine, Kasturba Medical College - Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India and Central Animal Research Facility, Manipal Academy of Higher Education, Manipal, Karnataka, India. We also thank Dr. Prakash P Y., Associate Professor, Department of Microbiology, Kasturba Medical College – Manipal, Manipal Academy of Higher Education for the guidance and support in drafting this manuscript. Conflicts of Interest: “The authors declare no conflict of interest.”
Publisher Copyright:
© 2022. Brazilian Archives of Biology and Technology.All Rights Reserved
PY - 2022
Y1 - 2022
N2 - Radioactive trivalent chromium (51Cr3+) is a known radiopharmaceutical used to tag plasma proteins, platelets and also for estimation of blood volume. Nevertheless, there exist insufficient reports with limited sample sizes concerning its clearance from blood and its biodistribution after intravenous administration. This study focused to understand clearance rate of 51Cr3+ from blood and analyze its biodistribution. For biodistribution, six adult New Zealand white albino rabbits were injected with 51Cr3+ through their marginal vein. Percentage clearance of 51Cr3+ from blood was calculated by recording radioactive counts obtained at 1, 58, 61, 120, 180 and 240 minutes post-administration in thirty-three adult New Zealand white albino rabbits. For evaluating 51Cr3+ biodistribution, organs were surgically removed from the rabbits and weighed. Radioactivity of the organs and urine were counted in a nucleonix gamma-ray spectrometer with a NaI scintillation detector. Data were expressed as cps/g. Average clearance of 51Cr3+ was 34% from the first to the 58 minute. Subsequent measurements for hourly clearance at 120, 180 and 240 minutes showed percentage reduction of radioactivity of 33, 14 and 8, respectively. Minimal specific activities were found in the muscle and brain. Spleen, lungs, liver and kidneys exhibited moderate radioactivity. Urine tracer-concentrations were found to be ten times more than that of plasma. From this study, it has been observed that clearance of 51Cr3+ from blood was faster initially which slowed down progressively and there displayed moderate uptake of 51Cr3+ by certain organs. Understanding pharmacokinetics of 51Cr3+ is relevant for its potential use as a diagnostic tool.
AB - Radioactive trivalent chromium (51Cr3+) is a known radiopharmaceutical used to tag plasma proteins, platelets and also for estimation of blood volume. Nevertheless, there exist insufficient reports with limited sample sizes concerning its clearance from blood and its biodistribution after intravenous administration. This study focused to understand clearance rate of 51Cr3+ from blood and analyze its biodistribution. For biodistribution, six adult New Zealand white albino rabbits were injected with 51Cr3+ through their marginal vein. Percentage clearance of 51Cr3+ from blood was calculated by recording radioactive counts obtained at 1, 58, 61, 120, 180 and 240 minutes post-administration in thirty-three adult New Zealand white albino rabbits. For evaluating 51Cr3+ biodistribution, organs were surgically removed from the rabbits and weighed. Radioactivity of the organs and urine were counted in a nucleonix gamma-ray spectrometer with a NaI scintillation detector. Data were expressed as cps/g. Average clearance of 51Cr3+ was 34% from the first to the 58 minute. Subsequent measurements for hourly clearance at 120, 180 and 240 minutes showed percentage reduction of radioactivity of 33, 14 and 8, respectively. Minimal specific activities were found in the muscle and brain. Spleen, lungs, liver and kidneys exhibited moderate radioactivity. Urine tracer-concentrations were found to be ten times more than that of plasma. From this study, it has been observed that clearance of 51Cr3+ from blood was faster initially which slowed down progressively and there displayed moderate uptake of 51Cr3+ by certain organs. Understanding pharmacokinetics of 51Cr3+ is relevant for its potential use as a diagnostic tool.
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U2 - 10.1590/1678-4324-2022210199
DO - 10.1590/1678-4324-2022210199
M3 - Article
AN - SCOPUS:85135800766
SN - 1516-8913
VL - 65
JO - Brazilian Archives of Biology and Technology
JF - Brazilian Archives of Biology and Technology
M1 - e22210627
ER -