Estimation of the fibre-composition of verhoff-stained fascia using colour-image processing

Rohit Nayak; A. K. Pandey; Pramod Kumar; Ramesh R. Galigekere

doi:10.1504/IJMEI.2011.041231

Estimation of the fibre-composition of verhoff-stained fascia using colour-image processing

Rohit Nayak, A. K. Pandey, Pramod Kumar, Ramesh R. Galigekere^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

This paper reports the results of our work on assessing the composition of abdominal fascia based on microscopic images of specimen stained by Verhoff's method, using colour-image processing. Verhoff'1s method of staining results in collagen fibres appearing red, and elastic fibres and speckle-like artefacts appearing black (elastic fibres being darker), in a yellowish background. The basic idea involves segmenting the red-coloured collagen first, followed by segmenting the black elastic fibres. We use the YIQ colour model for the purpose. Collagen is extracted by thresholding a contrast-enhanced version of the I-image, and elastic fibres are extracted by thresholding the Y-image based on an empirically estimated threshold-value. Results of experiments on real microscopic images are encouraging.

Original language	English
Pages (from-to)	99-107
Number of pages	9
Journal	International Journal of Medical Engineering and Informatics
Volume	3
Issue number	2
DOIs	https://doi.org/10.1504/IJMEI.2011.041231
Publication status	Published - 07-2011

All Science Journal Classification (ASJC) codes

Medicine (miscellaneous)
Biomaterials
Biomedical Engineering
Health Informatics

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10.1504/IJMEI.2011.041231

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title = "Estimation of the fibre-composition of verhoff-stained fascia using colour-image processing",

abstract = "This paper reports the results of our work on assessing the composition of abdominal fascia based on microscopic images of specimen stained by Verhoff's method, using colour-image processing. Verhoff'1s method of staining results in collagen fibres appearing red, and elastic fibres and speckle-like artefacts appearing black (elastic fibres being darker), in a yellowish background. The basic idea involves segmenting the red-coloured collagen first, followed by segmenting the black elastic fibres. We use the YIQ colour model for the purpose. Collagen is extracted by thresholding a contrast-enhanced version of the I-image, and elastic fibres are extracted by thresholding the Y-image based on an empirically estimated threshold-value. Results of experiments on real microscopic images are encouraging.",

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AU - Pandey, A. K.

AU - Kumar, Pramod

AU - Galigekere, Ramesh R.

PY - 2011/7

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N2 - This paper reports the results of our work on assessing the composition of abdominal fascia based on microscopic images of specimen stained by Verhoff's method, using colour-image processing. Verhoff'1s method of staining results in collagen fibres appearing red, and elastic fibres and speckle-like artefacts appearing black (elastic fibres being darker), in a yellowish background. The basic idea involves segmenting the red-coloured collagen first, followed by segmenting the black elastic fibres. We use the YIQ colour model for the purpose. Collagen is extracted by thresholding a contrast-enhanced version of the I-image, and elastic fibres are extracted by thresholding the Y-image based on an empirically estimated threshold-value. Results of experiments on real microscopic images are encouraging.

AB - This paper reports the results of our work on assessing the composition of abdominal fascia based on microscopic images of specimen stained by Verhoff's method, using colour-image processing. Verhoff'1s method of staining results in collagen fibres appearing red, and elastic fibres and speckle-like artefacts appearing black (elastic fibres being darker), in a yellowish background. The basic idea involves segmenting the red-coloured collagen first, followed by segmenting the black elastic fibres. We use the YIQ colour model for the purpose. Collagen is extracted by thresholding a contrast-enhanced version of the I-image, and elastic fibres are extracted by thresholding the Y-image based on an empirically estimated threshold-value. Results of experiments on real microscopic images are encouraging.

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Estimation of the fibre-composition of verhoff-stained fascia using colour-image processing

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