Estimation of the fibre-composition of verhoff-stained fascia using colour-image processing

Rohit Nayak, A. K. Pandey, Pramod Kumar, Ramesh R. Galigekere

Research output: Contribution to journalArticlepeer-review


This paper reports the results of our work on assessing the composition of abdominal fascia based on microscopic images of specimen stained by Verhoff's method, using colour-image processing. Verhoff'1s method of staining results in collagen fibres appearing red, and elastic fibres and speckle-like artefacts appearing black (elastic fibres being darker), in a yellowish background. The basic idea involves segmenting the red-coloured collagen first, followed by segmenting the black elastic fibres. We use the YIQ colour model for the purpose. Collagen is extracted by thresholding a contrast-enhanced version of the I-image, and elastic fibres are extracted by thresholding the Y-image based on an empirically estimated threshold-value. Results of experiments on real microscopic images are encouraging.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalInternational Journal of Medical Engineering and Informatics
Issue number2
Publication statusPublished - 07-2011

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Health Informatics


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