TY - JOUR
T1 - Ethambutol induces testicular damage and decreases the sperm functional competence in Swiss albino mice
AU - Rao, Arpitha
AU - Nayak, Guruprasad
AU - Kumari, Sandhya
AU - Prabhu, Amratha D.
AU - Khandige, Nalini
AU - Kalthur, Sneha Guruprasad
AU - Mutalik, Srinivas
AU - Kalthur, Guruprasad
AU - Adiga, Satish Kumar
PY - 2016/10/1
Y1 - 2016/10/1
N2 - The present study reports the effect of ethambutol (EMB) on testicular function. Prepubertal and adult male Swiss albino mice were treated with 40, 80, 160 mg/kg body weight of EMB, intraperitoneally, every alternate day for 4 weeks. After 2 weeks gap, mice were sacrificed to collect caudal spermatozoa. EMB treatment resulted in a dose-dependent decrease in the testicular weight, sperm count and motility while the percentage of sperm with head abnormalities, immature chromatin (P < 0.001) and DNA damage increased (P < 0.01). In addition, EMB treatment resulted in significant depletion of glutathione (P < 0.05–P < 0.01) and histopathological abnormalities such as large cells, vacuolation of tubules and isolated colonies of spermatogenic cells were observed. Oct4, 17β-Hsd and c-Kit mRNA was marginally elevated in EMB treated testes at the highest dose studied. In conclusion, the result of the present study indicates that EMB has adverse effect on testicular function and impairs the sperm functional competence.
AB - The present study reports the effect of ethambutol (EMB) on testicular function. Prepubertal and adult male Swiss albino mice were treated with 40, 80, 160 mg/kg body weight of EMB, intraperitoneally, every alternate day for 4 weeks. After 2 weeks gap, mice were sacrificed to collect caudal spermatozoa. EMB treatment resulted in a dose-dependent decrease in the testicular weight, sperm count and motility while the percentage of sperm with head abnormalities, immature chromatin (P < 0.001) and DNA damage increased (P < 0.01). In addition, EMB treatment resulted in significant depletion of glutathione (P < 0.05–P < 0.01) and histopathological abnormalities such as large cells, vacuolation of tubules and isolated colonies of spermatogenic cells were observed. Oct4, 17β-Hsd and c-Kit mRNA was marginally elevated in EMB treated testes at the highest dose studied. In conclusion, the result of the present study indicates that EMB has adverse effect on testicular function and impairs the sperm functional competence.
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U2 - 10.1016/j.etap.2016.08.012
DO - 10.1016/j.etap.2016.08.012
M3 - Article
C2 - 27579587
AN - SCOPUS:84983568190
SN - 1382-6689
VL - 47
SP - 28
EP - 37
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
ER -