Evaluation of Anti-inflammatory Potentials of Novel Flavonols and Their Metal Complexes

Synthesis of novel flavonols and their complexation with Zn, Fe, Cu and Co was carried out. The synthesized compounds were characterized by spectral methods. The characterized compounds were evaluated for their anti-oxidant activity and the test compounds exhibiting promising anti-oxidant activity were screened for their in-vitro anti-inflammatory activity by Cyclooxygenase (COX) and Lipoxygenase (LOX) inhibition assay. Among the synthesized compounds, three potential test compounds such as 3-(4-Benzyloxy-phenyl)-2-hydroxy-4H-naphthalen-1-one (F2), the zinc complex of F2, (F2M1) and 2-Hydroxy-3-(4-methylsulfanyl-phenyl)-4H-naphthalen-1-one (F4) were screened for their acute oral toxicity as per the Organization for Economic Co-operation and Development (OECD) guidelines and were found to be non-toxic to the animals at a dose of 2000mg/kg body weight. They were further evaluated for in-vivo anti-inflammatory activity both by carrageenan-induced acute paw edema method, carrageenan induced air-pouch model and Complete Freund's Adjuvant (CFA)-induced arthritis model.