TY - JOUR
T1 - Evaluation of antiarthritic potential and phytochemical analysis of different fractions of selected medicinal plants
AU - Mukhopadhyay, Nabarun
AU - Sampath, V.
AU - Pai, Sameer
AU - Babu, U. V.
AU - Lobo, Richard
N1 - Funding Information:
The authors are grateful to Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka and The Himalaya Drug Company, Karnataka for providing all infrastructure facility and support for carryout this work.
Publisher Copyright:
© 2021, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021
Y1 - 2021
N2 - Arthritis is a chronic inflammatory condition that is a major disability throughout the world. For so many years there are several therapies used for the treatment but they have many disadvantages like side effects, poor bioavailability, high cost etc. So, researches are going on for the development of alternative effective therapy for arthritis. The current study explores anti-arthritic activity (by targeting pro-inflammatory cytokines like IL-6) and phytochemical analysis of different fractions of Capsicum annum, Boswellia serrata and Lawsonia inermis. In this study overall seven fractions were prepared with the help of fractionation. They were coded as CO, COHF, COMF, BPS, HMEP, HMAP and HAMF. The anti-inflammatory activity of the fractions was evaluated by using an in-vitro IL-6 and TNF-ɑ ELISA study. The non-toxic concentration of the study samples was determined by MTT assay. High-PerformanceThin-Layer Chromatography was used for qualitative analysis of phytoconstituents present in the fractions. Liquid Chromatography-Mass Spectroscopy was used for the identification of phytoconstituents present in the fractions of COMF, BPS and HMEP. HPTLC analysis revealed the presence of capsaicin, galactose, arabinose, luteolin, β-sitosterol, LCMS analysis showed the presence of capsaicin, luteolin, lalioside and arabinose.
AB - Arthritis is a chronic inflammatory condition that is a major disability throughout the world. For so many years there are several therapies used for the treatment but they have many disadvantages like side effects, poor bioavailability, high cost etc. So, researches are going on for the development of alternative effective therapy for arthritis. The current study explores anti-arthritic activity (by targeting pro-inflammatory cytokines like IL-6) and phytochemical analysis of different fractions of Capsicum annum, Boswellia serrata and Lawsonia inermis. In this study overall seven fractions were prepared with the help of fractionation. They were coded as CO, COHF, COMF, BPS, HMEP, HMAP and HAMF. The anti-inflammatory activity of the fractions was evaluated by using an in-vitro IL-6 and TNF-ɑ ELISA study. The non-toxic concentration of the study samples was determined by MTT assay. High-PerformanceThin-Layer Chromatography was used for qualitative analysis of phytoconstituents present in the fractions. Liquid Chromatography-Mass Spectroscopy was used for the identification of phytoconstituents present in the fractions of COMF, BPS and HMEP. HPTLC analysis revealed the presence of capsaicin, galactose, arabinose, luteolin, β-sitosterol, LCMS analysis showed the presence of capsaicin, luteolin, lalioside and arabinose.
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U2 - 10.31788/RJC.2021.1415986
DO - 10.31788/RJC.2021.1415986
M3 - Article
AN - SCOPUS:85102852563
SN - 0974-1496
VL - 14
SP - 212
EP - 220
JO - Rasayan Journal of Chemistry
JF - Rasayan Journal of Chemistry
IS - 1
ER -