TY - JOUR
T1 - Evaluation of Gestational Diabetes Mellitus Risk in South Indian Women Based on MTHFR (C677T) and FVL (G1691A) Mutations
AU - Khan, Imran Ali
AU - Shaik, Noor Ahmad
AU - Kamineni, Vasundhara
AU - Jahan, Parveen
AU - Hasan, Qurratulain
AU - Rao, Pragna
N1 - Funding Information:
Gratitude is expressed to all the volunteers who have participated in this study. We are grateful to Indian Council for Medical Research for providing SRF to IAK and funding of this research (Sanction no. 5-3-8-39-2007; RHN).
Publisher Copyright:
Copyright © 2015 Khan, Shaik, Kamineni, Jahan, Hasan and Rao.
PY - 2015/5/5
Y1 - 2015/5/5
N2 - We aimed to scrutinize the extent to which single amino acid substitutions in the MTHFR and factor V Leiden (FVL) genes affect the risk of gestational diabetes mellitus (GDM) in pregnant women of South Indian descendant. This case–control study was implemented once the ethical approval has been obtained. Overall, 237 women were recruited in this study: 137 had been diagnosed with GDM and the remaining 100 women were used as normal controls or non-GDM. The diagnosis of GDM was confirmed with biochemical analysis, i.e., GCT and oral glucose tolerance tests. Five milliliters of peripheral blood was collected and used for biochemical and molecular analyses. DNA was isolated, and genotyping for MTHFR (C677T) and FVL (G1691A) mutations was performed using PCR–RFLP. FVL (G1691A) locus was not polymorphic in the investigated sample. There was no significant difference in the allele and genotype frequencies of C677T polymorphism between GDM and non-GDM women (p = 0.8892).
AB - We aimed to scrutinize the extent to which single amino acid substitutions in the MTHFR and factor V Leiden (FVL) genes affect the risk of gestational diabetes mellitus (GDM) in pregnant women of South Indian descendant. This case–control study was implemented once the ethical approval has been obtained. Overall, 237 women were recruited in this study: 137 had been diagnosed with GDM and the remaining 100 women were used as normal controls or non-GDM. The diagnosis of GDM was confirmed with biochemical analysis, i.e., GCT and oral glucose tolerance tests. Five milliliters of peripheral blood was collected and used for biochemical and molecular analyses. DNA was isolated, and genotyping for MTHFR (C677T) and FVL (G1691A) mutations was performed using PCR–RFLP. FVL (G1691A) locus was not polymorphic in the investigated sample. There was no significant difference in the allele and genotype frequencies of C677T polymorphism between GDM and non-GDM women (p = 0.8892).
UR - http://www.scopus.com/inward/record.url?scp=84971227564&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84971227564&partnerID=8YFLogxK
U2 - 10.3389/fped.2015.00034
DO - 10.3389/fped.2015.00034
M3 - Article
AN - SCOPUS:84971227564
SN - 2296-2360
VL - 3
JO - Frontiers in Pediatrics
JF - Frontiers in Pediatrics
M1 - 34
ER -
