TY - JOUR
T1 - Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
AU - Bhat, Samatha
AU - Viswanathan, Pachaiyappan
AU - Chandanala, Shashank
AU - Prasanna, S. Jyothi
AU - Seetharam, Raviraja N.
N1 - Funding Information:
This study was fully funded by Stempeutics Research Pvt Ltd, India. The authors thank RoosterBio for providing RoosterNourish and RoosterNourish-MSC XF medium, Miltenyi Biotec for providing StemMACS-MSC XF, Merck for providing PLTMax hPL, Biological Industries for providing MSC NutriStem XF and R&D Systems for providing StemXVivo SFM free of cost for evaluation purpose. The authors also thank Mrs. Neema Shetty and Mrs. Sharmila Singh for helping in the experimental set-up and Dr. Charan Thej for co-ordinating for the study related to qRT-PCR and functional assays.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are gaining increasing importance in the field of regenerative medicine. Although therapeutic value of MSCs is now being established through many clinical trials, issues have been raised regarding their expansion as per regulatory guidelines. Fetal bovine serum usage in cell therapy poses difficulties due to its less-defined, highly variable composition and safety issues. Hence, there is a need for transition from serum-based to serum-free media (SFM). Since SFM are cell type-specific, a precise analysis of the properties of MSCs cultured in SFM is required to determine the most suitable one. Six different commercially available low serum/SFM with two different seeding densities were evaluated to explore their ability to support the growth and expansion of BM-MSCs and assess the characteristics of BM-MSCs cultured in these media. Except for one of the SFM, all other media tested supported the growth of BM-MSCs at a low seeding density. No significant differences were observed in the expression of MSC specific markers among the various media tested. In contrary, the population doubling time, cell yield, potency, colony-forming ability, differentiation potential, and immunosuppressive properties of MSCs varied with one another. We show that SFM tested supports the growth and expansion of BM-MSCs even at low seeding density and may serve as possible replacement for animal-derived serum.
AB - Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are gaining increasing importance in the field of regenerative medicine. Although therapeutic value of MSCs is now being established through many clinical trials, issues have been raised regarding their expansion as per regulatory guidelines. Fetal bovine serum usage in cell therapy poses difficulties due to its less-defined, highly variable composition and safety issues. Hence, there is a need for transition from serum-based to serum-free media (SFM). Since SFM are cell type-specific, a precise analysis of the properties of MSCs cultured in SFM is required to determine the most suitable one. Six different commercially available low serum/SFM with two different seeding densities were evaluated to explore their ability to support the growth and expansion of BM-MSCs and assess the characteristics of BM-MSCs cultured in these media. Except for one of the SFM, all other media tested supported the growth of BM-MSCs at a low seeding density. No significant differences were observed in the expression of MSC specific markers among the various media tested. In contrary, the population doubling time, cell yield, potency, colony-forming ability, differentiation potential, and immunosuppressive properties of MSCs varied with one another. We show that SFM tested supports the growth and expansion of BM-MSCs even at low seeding density and may serve as possible replacement for animal-derived serum.
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U2 - 10.1038/s41598-021-83088-1
DO - 10.1038/s41598-021-83088-1
M3 - Article
C2 - 33564114
AN - SCOPUS:85100715503
SN - 2045-2322
VL - 11
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 3403
ER -