TY - JOUR
T1 - Experimentally Induced Hyperglycemia in Prepubertal Phase Impairs Oocyte Quality and Functionality in Adult Mice
AU - Predheepan, Dhakshanya
AU - Daddangadi, Akshatha
AU - Uppangala, Shubhashree
AU - Laxminarayana, Sindhura Lakshmi Koulmane
AU - Raval, Keyur
AU - Kalthur, Guruprasad
AU - Kovačič, Borut
AU - Kumar Adiga, Satish
N1 - Publisher Copyright:
© 2022 The Author(s). Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved.
PY - 2022/9/1
Y1 - 2022/9/1
N2 - Reproductive abnormalities in women with a history of childhood diabetes are believed to be partially attributed to hyperglycemia. Prolonged hyperglycemia can negatively affect ovarian function and fertility during reproductive life. To address this in an experimental setting, the present study used streptozotocin-induced hyperglycemic prepubertal mouse model. The impact of prolonged hyperglycemic exposure during prepubertal life on ovarian function, oocyte quality, and functional competence was assessed in adult mice. The ovarian reserve was not significantly altered; however, the in vitro maturation potential (P<0.001), mitochondrial integrity (P<0.01), and meiotic spindle assembly (P<0.05-0.001) in oocytes were significantly affected in hyperglycemic animals in comparison to control groups. The results from the study suggest that prepubertal hyperglycemia can have adverse effects on the oocyte functional competence and spindle integrity during the reproductive phase of life. Because these changes can have a significant impact on the genetic integrity and developmental potential of the embryos and fetus, the observation warrants further research both in experimental and clinical settings.
AB - Reproductive abnormalities in women with a history of childhood diabetes are believed to be partially attributed to hyperglycemia. Prolonged hyperglycemia can negatively affect ovarian function and fertility during reproductive life. To address this in an experimental setting, the present study used streptozotocin-induced hyperglycemic prepubertal mouse model. The impact of prolonged hyperglycemic exposure during prepubertal life on ovarian function, oocyte quality, and functional competence was assessed in adult mice. The ovarian reserve was not significantly altered; however, the in vitro maturation potential (P<0.001), mitochondrial integrity (P<0.01), and meiotic spindle assembly (P<0.05-0.001) in oocytes were significantly affected in hyperglycemic animals in comparison to control groups. The results from the study suggest that prepubertal hyperglycemia can have adverse effects on the oocyte functional competence and spindle integrity during the reproductive phase of life. Because these changes can have a significant impact on the genetic integrity and developmental potential of the embryos and fetus, the observation warrants further research both in experimental and clinical settings.
UR - http://www.scopus.com/inward/record.url?scp=85136006823&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136006823&partnerID=8YFLogxK
U2 - 10.1210/endocr/bqac121
DO - 10.1210/endocr/bqac121
M3 - Article
C2 - 35917567
AN - SCOPUS:85136006823
SN - 0013-7227
VL - 163
JO - Endocrinology
JF - Endocrinology
IS - 9
M1 - bqac121
ER -