Expression of PTCH gene in ameloblastoma and odontogenic keratocyst: A comparative study

Sharada Srinagesh, N. Sahana, G. Suganya, S. Renuga, Srikant Natarajan, Meghashyama Kulkarni

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: This is a cross-sectional comparative study, aimed to quantify the expression of patched (PTCH) gene in ameloblastoma, odontogenic keratocyst (OKC) and also the comparison of both the expressions. Materials and Methods: Genomic deoxyribonucleic acid (DNA) was extracted and quantified, and the expression of the PTCH gene was done in 17 cases of ameloblastoma and 17 cases of OKC by quantitative real-time polymerase chain reaction (RT-qPCR). Results: It was observed that there was an overexpression of the PTCH gene in both ameloblastoma and OKC with a good mean cycle threshold (CT) value of 32.71 ± 2.432 and 34.69 ± 1.875, respectively. When comparing the PTCH expression between the two, ameloblastoma showed higher expression than the OKC and the difference is statistically significant with P value of 0.025. Conclusions: Our findings suggest that there is overexpression of PTCH in ameloblastoma and OKC, but it is highly expressed in ameloblastoma when compared to OKC. Overexpression of PTCH may constitute the activation of the Sonic Hedgehog pathway and may suggest the mechanism for the development of ameloblastoma and OKC. Hence it can be used as a valuable marker for early diagnosis and in the identification of therapeutic targets.

Original languageEnglish
Pages (from-to)427-428
Number of pages2
JournalJournal of Oral and Maxillofacial Pathology
Volume27
Issue number2
DOIs
Publication statusPublished - 04-2023

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Dentistry(all)

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