Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation

Sangeeta Nath; Manli Deng; Yves Engelborghs

doi:10.1007/978-1-61779-927-3_29

Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation

Sangeeta Nath, Manli Deng, Yves Engelborghs

Manipal Institute of Regenerative Medicine, Bangalore

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Citations (Scopus)

Abstract

Fluorescence correlation spectroscopy (FCS) can be used to determine the diffusion coefficient of fluorescently labeled α-synuclein. It is a technique based on the use of a confocal microscope. By applying FCS in a combination of short sampling times and repeated measurements, the disappearance of individual α-synuclein molecules (called monomers) and the formation of oligomers can be characterized during the early aggregation process.

Original language	English
Title of host publication	Intrinsically Disordered Protein Analysis
Subtitle of host publication	Volume 1, Methods and Experimental Tools
Editors	Vladimir Uversky, Vladimir Uversky, Keith Dunker
Pages	499-506
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-61779-927-3_29
Publication status	Published - 2012

Publication series

Name	Methods in Molecular Biology
Volume	895
ISSN (Print)	1064-3745

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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10.1007/978-1-61779-927-3_29

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Nath, S., Deng, M., & Engelborghs, Y. (2012). Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation. In V. Uversky, V. Uversky, & K. Dunker (Eds.), Intrinsically Disordered Protein Analysis: Volume 1, Methods and Experimental Tools (pp. 499-506). (Methods in Molecular Biology; Vol. 895). https://doi.org/10.1007/978-1-61779-927-3_29

Nath, Sangeeta ; Deng, Manli ; Engelborghs, Yves. / Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation. Intrinsically Disordered Protein Analysis: Volume 1, Methods and Experimental Tools. editor / Vladimir Uversky ; Vladimir Uversky ; Keith Dunker. 2012. pp. 499-506 (Methods in Molecular Biology).

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abstract = "Fluorescence correlation spectroscopy (FCS) can be used to determine the diffusion coefficient of fluorescently labeled α-synuclein. It is a technique based on the use of a confocal microscope. By applying FCS in a combination of short sampling times and repeated measurements, the disappearance of individual α-synuclein molecules (called monomers) and the formation of oligomers can be characterized during the early aggregation process.",

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Nath, S, Deng, M & Engelborghs, Y 2012, Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation. in V Uversky, V Uversky & K Dunker (eds), Intrinsically Disordered Protein Analysis: Volume 1, Methods and Experimental Tools. Methods in Molecular Biology, vol. 895, pp. 499-506. https://doi.org/10.1007/978-1-61779-927-3_29

Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation. / Nath, Sangeeta; Deng, Manli; Engelborghs, Yves.
Intrinsically Disordered Protein Analysis: Volume 1, Methods and Experimental Tools. ed. / Vladimir Uversky; Vladimir Uversky; Keith Dunker. 2012. p. 499-506 (Methods in Molecular Biology; Vol. 895).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

TY - CHAP

T1 - Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation

AU - Nath, Sangeeta

AU - Deng, Manli

AU - Engelborghs, Yves

PY - 2012

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AB - Fluorescence correlation spectroscopy (FCS) can be used to determine the diffusion coefficient of fluorescently labeled α-synuclein. It is a technique based on the use of a confocal microscope. By applying FCS in a combination of short sampling times and repeated measurements, the disappearance of individual α-synuclein molecules (called monomers) and the formation of oligomers can be characterized during the early aggregation process.

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Nath S, Deng M, Engelborghs Y. Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation. In Uversky V, Uversky V, Dunker K, editors, Intrinsically Disordered Protein Analysis: Volume 1, Methods and Experimental Tools. 2012. p. 499-506. (Methods in Molecular Biology). doi: 10.1007/978-1-61779-927-3_29

Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation

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