TY - JOUR
T1 - Fluorescence In Situ hybridization (FISH) assays for diagnosing malaria in endemic areas
AU - Shah, Jyotsna
AU - Mark, Olivia
AU - Weltman, Helena
AU - Barcelo, Nicolas
AU - Lo, Wai
AU - Wronska, Danuta
AU - Kakkilaya, Srinivas
AU - Rao, Aravinda
AU - Bhat, Shalia T.
AU - Sinha, Ruchi
AU - Omar, Sabah
AU - O'bare, Peter
AU - Moro, Manuel
AU - Gilman, Robert H.
AU - Harris, Nick
PY - 2015/9/2
Y1 - 2015/9/2
N2 - Malaria is a responsible for approximately 600 thousand deaths worldwide every year. Appropriate and timely treatment of malaria can prevent deaths but is dependent on accurate and rapid diagnosis of the infection. Currently, microscopic examination of the Giemsa stained blood smears is the method of choice for diagnosing malaria. Although it has limited sensitivity and specificity in field conditions, it still remains the gold standard for the diagnosis of malaria. Here, we report the development of a fluorescence in situ hybridization (FISH) based method for detecting malaria infection in blood smears and describe the use of an LED light source that makes the method suitable for use in resource-limited malaria endemic countries. The Plasmodium Genus (P-Genus) FISH assay has a Plasmodium genus specific probe that detects all five species of Plasmodium known to cause the disease in humans. The P. falciparum (PF) FISH assay and P. vivax (PV) FISH assay detect and differentiate between P. falciparum and P. vivax respectively from other Plasmodium species. The FISH assays are more sensitive than Giemsa. The sensitivities of P-Genus, PF and PV FISH assays were found to be 98.2%, 94.5% and 98.3%, respectively compared to 89.9%, 83.3%and 87.9% for the detection of Plasmodium, P. falciparum and P. vivax by Giemsa staining respectively.
AB - Malaria is a responsible for approximately 600 thousand deaths worldwide every year. Appropriate and timely treatment of malaria can prevent deaths but is dependent on accurate and rapid diagnosis of the infection. Currently, microscopic examination of the Giemsa stained blood smears is the method of choice for diagnosing malaria. Although it has limited sensitivity and specificity in field conditions, it still remains the gold standard for the diagnosis of malaria. Here, we report the development of a fluorescence in situ hybridization (FISH) based method for detecting malaria infection in blood smears and describe the use of an LED light source that makes the method suitable for use in resource-limited malaria endemic countries. The Plasmodium Genus (P-Genus) FISH assay has a Plasmodium genus specific probe that detects all five species of Plasmodium known to cause the disease in humans. The P. falciparum (PF) FISH assay and P. vivax (PV) FISH assay detect and differentiate between P. falciparum and P. vivax respectively from other Plasmodium species. The FISH assays are more sensitive than Giemsa. The sensitivities of P-Genus, PF and PV FISH assays were found to be 98.2%, 94.5% and 98.3%, respectively compared to 89.9%, 83.3%and 87.9% for the detection of Plasmodium, P. falciparum and P. vivax by Giemsa staining respectively.
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U2 - 10.1371/journal.pone.0136726
DO - 10.1371/journal.pone.0136726
M3 - Article
C2 - 26333092
AN - SCOPUS:84947760071
SN - 1932-6203
VL - 10
JO - PLoS One
JF - PLoS One
IS - 9
M1 - e0136726
ER -
