TY - GEN
T1 - Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission
AU - Ge, Jianhong
AU - Wang, Yixin
AU - Kuang, Cuifang
AU - Lee, Shin Shian
AU - Mazumder, Nirmal
AU - Kao, Fu Jen
PY - 2013
Y1 - 2013
N2 - We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.
AB - We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.
UR - https://www.scopus.com/pages/publications/84878706564
UR - https://www.scopus.com/pages/publications/84878706564#tab=citedBy
U2 - 10.1117/12.2009387
DO - 10.1117/12.2009387
M3 - Conference contribution
AN - SCOPUS:84878706564
SN - 9780819493576
VL - 8588
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Multiphoton Microscopy in the Biomedical Sciences XIII
T2 - Multiphoton Microscopy in the Biomedical Sciences XIII
Y2 - 3 February 2013 through 5 February 2013
ER -