TY - JOUR
T1 - Formulation and Characterization of RBCS Coated Carboplatin Loaded Nano-Liposomal Formulation for Managing Breast Cancer
AU - Dubey, Akhilesh
AU - Raju, Faby
AU - Lobo, Cynthia Lizzie
AU - Gs, Ravi
AU - Hebbar, Srinivas
AU - Shetty, Amitha
AU - Kumar, Pankaj
AU - El-Zahaby, Sally A.
N1 - Publisher Copyright:
© 2024 Wiley Periodicals LLC.
PY - 2024/12
Y1 - 2024/12
N2 - Cell membrane-coated Nano-Liposomes (CM-NLPs) offer a promising approach that combines the advantages of both host cells and synthetic nano-liposomes (NLPs). This technique involves coating liposomes with red blood cell (RBC) membranes to enhance their functionality. In this study, novel carboplatin-loaded NLPs (CP-NLPs) were formulated using phospholipids (Soya Phosphatidyl Choline) and cholesterol through the thin-film hydration method, and optimized using a 32 full factorial design. The optimized CP-NLPs were coated with RBC membranes, resulting in the formulation “CP-RBCs-NLPs.” These were characterized for particle size, zeta potential, entrapment efficiency, transmission electron microscopy (TEM), differential scanning calorimetry (DSC), protein content, in vitro drug release, cell viability, and stability. The optimized CP-RBCs-NLPs exhibited a particle size of 103.6 nm, with zeta potential values of −27.3 mV indicating good stability. The entrapment efficiency was approximately 56%, and the drug release profile showed sustained release for up to 8 h. Cytotoxicity studies in human triple-negative breast cancer (MDA-MB468) cell lines demonstrated that CP-RBCs-NLPs effectively delivered the drug into target cells, facilitating cell death due to their bilayer structure similar to cell membranes. Overall, CP-RBCs-NLPs outperformed both carboplatin-loaded conventional NLPs (CP-CNLPs) and carboplatin-conventional solution (CP-CNS), making it a superior formulation for drug delivery.
AB - Cell membrane-coated Nano-Liposomes (CM-NLPs) offer a promising approach that combines the advantages of both host cells and synthetic nano-liposomes (NLPs). This technique involves coating liposomes with red blood cell (RBC) membranes to enhance their functionality. In this study, novel carboplatin-loaded NLPs (CP-NLPs) were formulated using phospholipids (Soya Phosphatidyl Choline) and cholesterol through the thin-film hydration method, and optimized using a 32 full factorial design. The optimized CP-NLPs were coated with RBC membranes, resulting in the formulation “CP-RBCs-NLPs.” These were characterized for particle size, zeta potential, entrapment efficiency, transmission electron microscopy (TEM), differential scanning calorimetry (DSC), protein content, in vitro drug release, cell viability, and stability. The optimized CP-RBCs-NLPs exhibited a particle size of 103.6 nm, with zeta potential values of −27.3 mV indicating good stability. The entrapment efficiency was approximately 56%, and the drug release profile showed sustained release for up to 8 h. Cytotoxicity studies in human triple-negative breast cancer (MDA-MB468) cell lines demonstrated that CP-RBCs-NLPs effectively delivered the drug into target cells, facilitating cell death due to their bilayer structure similar to cell membranes. Overall, CP-RBCs-NLPs outperformed both carboplatin-loaded conventional NLPs (CP-CNLPs) and carboplatin-conventional solution (CP-CNS), making it a superior formulation for drug delivery.
UR - https://www.scopus.com/pages/publications/85210007750
UR - https://www.scopus.com/inward/citedby.url?scp=85210007750&partnerID=8YFLogxK
U2 - 10.1002/ddr.70019
DO - 10.1002/ddr.70019
M3 - Article
C2 - 39558836
AN - SCOPUS:85210007750
SN - 0272-4391
VL - 85
JO - Drug Development Research
JF - Drug Development Research
IS - 8
M1 - e70019
ER -
