TY - JOUR
T1 - Free radical scavenging activity of aqueous root extract of Argyreia nervosa (Burm.f.) Boj. (Convolvulaceae)
AU - Shreedhara, C.S.
AU - Aswatha Ram, H.N.
AU - Zanwar, S.B.
AU - Falguni, G.P.
N1 - Cited By :3
Export Date: 10 November 2017
CODEN: JNROA
Correspondence Address: Shreedhara, C. S.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104, India; email: cssncp@rediffmail.com
Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), 28752-68-3; alpha tocopherol, 1406-18-4, 1406-70-8, 52225-20-4, 58-95-7, 59-02-9; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; hemoglobin, 9008-02-0; nitric oxide, 10102-43-9; superoxide, 11062-77-4
References: Shirwaikar, A., Shirwaikar, A., Punitha, I.S.R., (2007) Natural Product Sciences., 13 (1), pp. 40-45; Longman, O., (2003) Indian Medicinal Plants, a Compendium of 500 Species, 4, p. 264; Molyneux, P., (2004) Sci. and Technol., 26 (2), pp. 212-219; Schlesier, K., Harwart, M., Bohm, V., Bitsch, R., (2002) Free Radic.Res., 30 (2), pp. 177-185; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice Evans, C., (1998) Free Radic. Biol. Med., 72, pp. 1231-1237; Blois, (1958) Nature, 26, pp. 1199-1201; Benzie, I.F.F., Strain, J.T., (1996) Anal. Biochem., 239, pp. 70-76; Benzie, I.F.F., Szeto, Y.T., (1999) J. Agri. Food Chem., 47, pp. 633-636; Govindrarajan, R., Vijaykumar, M., Rawat, A.K.S., Mehrotra, S., (2003) Indian J. Exp. Biol., 41, p. 875; Muthunjaya, K., Hukkeri, V.I., (2008) PHCOG MAG., 4 (13), pp. 42-51; Sreejayan, N., Rao, M.N.A., (1997) J. Pharm. Pharmacol., 49, pp. 105-107. , a; Sreejayan, N., Rao, M.N.A., (1996) Drug Res., 46, pp. 169-172. , b; Shirwaikar, A., Rajendra, K., Dinesh Kumar, C., (2004) Indian J. Exp. Biol., 42, pp. 803-807; Preito, P., Pinedo, M., Aguilar, M., (1999) Anal. Biochem., 269, pp. 337-341; Pal, D.K., Dutta, S., (2006) Indian Journal of Pharmaceutical Sciences., 68 (2), pp. 256-258; Marx, J.L., (1987) Science., 235, pp. 512-529; Arora, A., Sairam, R.K., Srivastava, G.C., (2002) Cur. Sci., 82, pp. 1227-1229; Geesin, J.G., Gordon, J.S., Berg, R.A., (1990) Arch. Biochem. Biphy., 278, pp. 352-355; Badmi, S., Gupta, M.K., Suresh, B., (2003) J. Ethanopharmacol., 85, pp. 227-230; Sanchez-Morino, C., (2002) Food Sci. and Technol. Int., 8, pp. 122-126; Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H., (2004) Biol.Pharm. Bull., 27 (1), pp. 38-46; Chang, L.W., Yen, W.J., Huang, S.C., Duh, P.D., (2002) Food Chem., 78, pp. 347-354; Halliwell, B., (1991) Am. J. Med., 91, pp. 14S-22S; Duh, P.D., Tu, Y.Y., Yen, G.C., (1999) Lebensmittel-Wissenschaft and Technologie, 32, pp. 269-277; Gordon, M.H., The mechanism of the antioxidant action in vitro (1990) Food Antioxidants, pp. 1-18. , Hudson, BJF (Ed.), Ekseveir, London; Rice-Evans, C., Miller, N.J., (1997) Free Radic. Res., 195, pp. 26-27; Hussain, S.A., Ray, G., (2002) Indian J. Exp. Biol., 40, pp. 1213-1232; Ialenti, A., Moncada, S., Di Rosa, M., (1993) British J. Pharmacol., 362, pp. 801-805; Cross, A.R., Jones, O.T.G., (1991) Biophys. Acta., 387, pp. 281-285; Wiseman, H., Halliwell, B., (1996) Biochem. J., 313, pp. 17-19; Shirwaikar, A., Prabhu, K.S., Punitha, I.S.R., (2006) Indian J. Exp. Biol., 44, pp. 993-996
PY - 2009
Y1 - 2009
N2 - Aqueous extract of Argyreia nervosa, which is widely used in the indigenous system of medicine, was studied for its in vitro scavenging activity by different methods viz. DPPH radical scavenging, ABTS radical scavenging, lipid peroxidation, iron chelating activity, superoxide scavenging, total antioxidant capacity, Nitric oxide scavenging and Non-enzymatic Glycosylation of Haemoglobin assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 88.86 μg/ml for DPPH radical scavenging, 66.64 μg/ml for ABTS radical scavenging, 31.57 μg/ml for lipid peroxidation, 8.0 μg/ml for iron chelating activity, 113.20 μg/ml for Nitric oxide scavenging and 7.51 μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 22.0 μg/ml. In Haemoglobin glycosylation the % scavenging was found to be 43.77% and 64.42% for concentration 0.5 mg/ml. and 1.0 mg/ml respectively. In all the methods, the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Argyreia nervosa has significant antioxidant activity.
AB - Aqueous extract of Argyreia nervosa, which is widely used in the indigenous system of medicine, was studied for its in vitro scavenging activity by different methods viz. DPPH radical scavenging, ABTS radical scavenging, lipid peroxidation, iron chelating activity, superoxide scavenging, total antioxidant capacity, Nitric oxide scavenging and Non-enzymatic Glycosylation of Haemoglobin assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 88.86 μg/ml for DPPH radical scavenging, 66.64 μg/ml for ABTS radical scavenging, 31.57 μg/ml for lipid peroxidation, 8.0 μg/ml for iron chelating activity, 113.20 μg/ml for Nitric oxide scavenging and 7.51 μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 22.0 μg/ml. In Haemoglobin glycosylation the % scavenging was found to be 43.77% and 64.42% for concentration 0.5 mg/ml. and 1.0 mg/ml respectively. In all the methods, the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Argyreia nervosa has significant antioxidant activity.
M3 - Article
SN - 0972-5547
VL - 9
SP - 216
EP - 223
JO - Journal of Natural Remedies
JF - Journal of Natural Remedies
IS - 2
ER -