@article{31fd3076c3b94e0c933b2aa3460cb6c0,
title = "Free radical scavenging and lipid peroxidation inhibition potential of Hygrophila auriculata",
abstract = "Hygrophila auriculata (K. Schum.) Heine is a wild herb commonly found in moist places on the banks of tanks, ditches and paddy fields throughout India and is one of the main sources of Ayurvedic 'Rasayana' drug. The free radical scavenging potential of 50% aqueous alcoholic extract of H. auriculata (HAEt) was studied for DPPH scavenging activity, nitric oxide, hydroxyl radical and ferryl bipyridyl complex scavenging activity along with lipid peroxidation and total antioxidant capacity inhibition using Thiobarbituric acid reactive substances (TBARS) in rat liver homogenate. Integral antioxidative capacity was determined by photochemiluminescence assay. HAEt showed good radical scavenging activity at various concentrations (200-1000 μg/ml) against 1,1 diphenyl, 2-picrylhydrazyl (DPPH) (32.32-77.02%) with moderate scavenging activity against Nitric oxide (12.46-52.84%), hydroxyl radical (11.69-55.26%), ferryl bipyridyl complex (17.66-58.67%) and lipid peroxidation (0.829-0.416 nmoles/mg protein). The above results indicate HAEt to be very effective antioxidant. It was suggested that the varied therapeutic activities claimed for the plant in the Indian indigenous systems of medicine may be in part due to the free radical scavenging and/or potent antioxidant activity.",
author = "M. Vijayakumar and R. Govindarajan and A. Shirwaikar and V. Kumar and A.K.S. Rawat and S. Mehrotra and P. Pushpangadan",
note = "Cited By :10 Export Date: 10 November 2017 CODEN: NPSCF Correspondence Address: Pushpangadan, P.; Pharmacog. and Ethnopharmacol. Div., Natl. Botanical Research Institute, Lucknow 226-001, India; email: pushpangadan@satyam.net.in Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; hydroxyl radical, 3352-57-6; nitric oxide, 10102-43-9 References: Blois, M.S., Antioxidant determinations by the use of stable free radical (1958) Nature, 26, p. 1199; Delanty, N., Dichter, M.A., Oxidative injury in the nervous system (1998) Acta Nurologica Scandinavica, 98, pp. 145-153; Desmarchelier, C., Ramao, R.L., Coussio, J., Ciccia, G., Antioxidant and free radical scavenging activities in extracts from medicinal trees used in the Caatinga region in northeastern Brazil (1999) J. Ethnopharmacol., 67, pp. 69-77; Fernando, M.R., Wickramasinghe, S.M.D., Thabrew, M.I., Karunanayaka, E.H., A preliminary investigation of the possible hypoglycaemic activity of Asteracanthus longifolia (1989) J. Ethnopharmacol., 27, pp. 7-14; Govindarajan, R., Rastogi, S., Vijayakumar, M., Rawat, A.K.S., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Studies on the antioxidant activities of Desmodium gangeticum (2003) Biol. Pharm. Bull., 26, pp. 1424-1427; Govindarajan, R., Vijayakumar, M., Rao, Ch.V., Rawat, A.K.S., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Antioxidant potential of Anogeissus latifolia (2004) Biol. Pharm. Bull., 27, pp. 1266-1269; Govindarajan, R., Vijayakumar, M., Rao, Ch.V., Kumar, V., Rawat, A.K.S., Pushpangadan, P., Action of Asparagus racemosus against streptozotocin -induced oxidative stress (2004) Nat. Prod. Sci., 10, pp. 177-181; Govindarajan, R., Vijayakumar, M., Rawat, A.K.S., Mehrotra, S., Free radical scavenging potential of Picrorhiza kurrooa Royle ex Benth (2003) Indian J. Exp. Biol., 41, pp. 875-879; Gutteridge, J.M.C., Age pigments and free radicals: Fluorescent lipid complexes formed by iron and copper containing proteins (1985) Biochem. Biophy. Acta, 834, p. 144; Halliwell, B., Gutteridge, J.M.C., Free radicals, aging and diseases (1989) Free Radicals in Biology and Medicine, pp. 419-422. , Oxford press, Oxford; Halliwell, B., Superoxide-dependent formation of hydroxyl radicals in the presence of iron chelates (1978) FEBS Letters, 92, pp. 321-326; Jayatilak, P.G., Pardanani, D.S., Murty, B.D., Seth, A.R., Effect of an indigenous drug (speman) on accessory reproductive fucntions of mice (1976) Indian J. Exp. Biol., 14, pp. 170-173; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.I., Protein determination using Folin-ciocalteau reagent (1951) J. Biol. Chem., 193, pp. 438-448; Mariette, M.A., Nitric oxide: Biosynthesis and Biological significance (1989) Trend. Biochem. Sci., 14, p. 488; Miller, M.J., Sadowska-Krowicka, H., Chotinaruemol, S., Kakkis, J.L., Clark, D.A., Amelioration of chronic ileitis by nitric oxide synthase inhibition (1992) J. Pharmacol. Exp. Ther., 264, pp. 11-16; Nash, T., The colorimetric estimation of formaldehyde by means of the Hantzsch reaction (1953) Biochem. J., 55, p. 416; Okhawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal. Biochem., 95, p. 351; Preito, P., Pineda, M., Aguilar, M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phophomolybdemnum complex: Specific application to the determination of vitamin E (1999) Anal. Biochem., 269, pp. 337-341; Singh, A., Handa, S.S., Hepatoprotective activity of graveolens and H. auriculata against Paracetamol and thioacetamide intoxication in rats (1999) J. Ethnopharmacol., 49, pp. 119-126; Sreejayan, N., Rao, M.N.A., Nitric oxide scavenging by curcuminoids (1997) J. Pharm. Pharmacol., 49, p. 105; Thomas, C.E., Kalayanaram, B., (1999) Oxygen Radicals and the Disease Process, 3rd Ed., , Hardwood Academic Publishers, Netherland; Tripathi, Y.B., Sharma, M., Comparison of the antioxidant action of the alcoholic extract of Rubia cordifolia with Rubiadin (1998) Indian J. Biochem. Biophys., 35, p. 313; Valentao, P., Fernndes, E., Canvalho, E., Andrade, P.B., Seabra, R.M., Bastos, M.L., Antioxidant activity of Hypericum androsaenium infusion scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid (2002) Biol. Pharm. Bull., 25, pp. 1324-1327; Wiseman, H., Halliwell, B., Damage to DNA by reactive oxygen and nitrogen species: Role in inflammatory disease and progression to cancer (1996) Biochem. J., 313, pp. 17-29",
year = "2005",
language = "English",
volume = "11",
pages = "22--26",
journal = "Natural Product Sciences",
issn = "1226-3907",
publisher = "Korean Society of Pharmacognosy",
number = "1",
}