TY - JOUR
T1 - Gabapentin loaded silver nanoparticles (GBP@AgNPs) for its promising biomedical application as a nanodrug
T2 - Anticancer and Antimicrobial activities
AU - Yaseen, Bushra
AU - Gangwar, Chinky
AU - Nayak, Rashmi
AU - Kumar, Saurabh
AU - Sarkar, Joy
AU - Banerjee, Monisha
AU - Naik, Radhey Mohan
N1 - Funding Information:
The authors are grateful to the HOD, Chemistry, Lucknow University, Lucknow (U.P.) India, for providing basic infrastructure facilities, FT-IR spectroscopy, and UV-visible spectrophotometry for performing experimental work. The authors are also thankful to Vice-Chancellor Prof. Alok Rai of Lucknow University for partially assisting with funds through the Award of Karmyogi scholarship to B.Y. funded by University of Lucknow, India. The authors are also grateful to the IIT Mandi, advanced materials research centre (AMRC), Kamand campus, H.P, and All India Institute of Medical Science (AIIMS) New Delhi, India, for the sophisticated analytical instrumentation facility (SAIF), for providing an X-ray diffraction, field emission scanning electron microscopy and transmission electron microscopy facilities respectively. The authors are grateful to the Director, CSIR- Central Drug Research Institute, Lucknow, Uttar Pradesh India, for the biological assay facility. The authors are indebted to the Centre of Excellence scheme, Government of Uttar Pradesh, India, for cell culture facility in Molecular and Human Genetics Laboratory, Department of Zoology, University of Lucknow, Lucknow.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2023/3
Y1 - 2023/3
N2 - Herein, we report the effectiveness of the gabapentin loaded silver nanoparticles (GBP@AgNPs) prepared involving gabapentin (GBP) which acts as both a reducing as well as a stabilizing agent at 35 °C. The prepared samples were subjected to different characterisations, including Ultraviolet–Visible Spectrophotometry (UV–vis), fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy-dispersive X-ray spectroscopy (EDAX), and powder X-ray diffraction techniques (pXRD). The characterisation techniques corroborate that the prepared silver nanoparticles loaded with gabapentin are in the nano range (20–25 nm) with a face-centred cubic (fcc) crystal structure. The biological effectiveness in terms of cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide tetrazolium dye (MTT) assay against human lung carcinoma cells A-549. The Half Maximum Inhibitory Concentration (IC50) value was observed at ∼ 150 μM in vitro. For the antibacterial potential, the minimal inhibition concentration (MIC) for the prepared (GBP@AgNPs) was found to be excellent for gram negative bacteria Klebsiella pneumonia at 16.0 µg/ml compared with the standard drug Levofloxacin having MIC value 64.0 µg/ml and for Acinetobacter baumannii 4.0 µg/ml with the standard drug Levofloxacin (8.0 µg/ml). Moreover, the best result for antimycotic activity of the prepared (GBP@AgNPs) having MIC value 6.25 µg/ml for C. Parapsilosis compared with the standard drug Fluconazole (2.0 µg/ml). The result suggests that GBP@AgNPs can be effectively used as an anticancerous, antibacterial and antimycotic drug and compatible with potential future nanodrug which can have immense potential for drug delivery in the near future.
AB - Herein, we report the effectiveness of the gabapentin loaded silver nanoparticles (GBP@AgNPs) prepared involving gabapentin (GBP) which acts as both a reducing as well as a stabilizing agent at 35 °C. The prepared samples were subjected to different characterisations, including Ultraviolet–Visible Spectrophotometry (UV–vis), fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy-dispersive X-ray spectroscopy (EDAX), and powder X-ray diffraction techniques (pXRD). The characterisation techniques corroborate that the prepared silver nanoparticles loaded with gabapentin are in the nano range (20–25 nm) with a face-centred cubic (fcc) crystal structure. The biological effectiveness in terms of cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide tetrazolium dye (MTT) assay against human lung carcinoma cells A-549. The Half Maximum Inhibitory Concentration (IC50) value was observed at ∼ 150 μM in vitro. For the antibacterial potential, the minimal inhibition concentration (MIC) for the prepared (GBP@AgNPs) was found to be excellent for gram negative bacteria Klebsiella pneumonia at 16.0 µg/ml compared with the standard drug Levofloxacin having MIC value 64.0 µg/ml and for Acinetobacter baumannii 4.0 µg/ml with the standard drug Levofloxacin (8.0 µg/ml). Moreover, the best result for antimycotic activity of the prepared (GBP@AgNPs) having MIC value 6.25 µg/ml for C. Parapsilosis compared with the standard drug Fluconazole (2.0 µg/ml). The result suggests that GBP@AgNPs can be effectively used as an anticancerous, antibacterial and antimycotic drug and compatible with potential future nanodrug which can have immense potential for drug delivery in the near future.
UR - https://www.scopus.com/pages/publications/85145992514
UR - https://www.scopus.com/pages/publications/85145992514#tab=citedBy
U2 - 10.1016/j.inoche.2022.110380
DO - 10.1016/j.inoche.2022.110380
M3 - Article
AN - SCOPUS:85145992514
SN - 1387-7003
VL - 149
JO - Inorganic Chemistry Communications
JF - Inorganic Chemistry Communications
M1 - 110380
ER -