TY - JOUR
T1 - Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines
AU - Kanafi, Mohammad Mahboob
AU - Mamidi, Murali Krishna
AU - Sureshbabu, Shalini Kashipathi
AU - Shahani, Pradnya
AU - Bhawna, Chandravanshi
AU - Warrier, Sudha R.
AU - Bhonde, Ramesh
N1 - Publisher Copyright:
© 2014, Springer Science+Business Media Dordrecht.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation protocol which gave two distinct cell populations: single cells and cellular aggregates. Subsequent analysis confirmed their identity as pancreatic acinar cells and islet-like cell aggregates (ICAs), as evidenced by amylase secretion and diphenylthiocarbazone staining respectively. Reverse transcriptase-PCR and immunocytochemistry assessment of the ICAs revealed the expression of pancreatic specific markers Ngn-3, Glut-2, Pax-6 and Isl-1. These ICAs secreted insulin in response to glucose challenge, confirming their functionality. We propose that ICAs generated from HeLa and MCF-7 cell lines could form a promising in vitro platform of human islet equivalents (hIEQs) for diabetes research.
AB - To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation protocol which gave two distinct cell populations: single cells and cellular aggregates. Subsequent analysis confirmed their identity as pancreatic acinar cells and islet-like cell aggregates (ICAs), as evidenced by amylase secretion and diphenylthiocarbazone staining respectively. Reverse transcriptase-PCR and immunocytochemistry assessment of the ICAs revealed the expression of pancreatic specific markers Ngn-3, Glut-2, Pax-6 and Isl-1. These ICAs secreted insulin in response to glucose challenge, confirming their functionality. We propose that ICAs generated from HeLa and MCF-7 cell lines could form a promising in vitro platform of human islet equivalents (hIEQs) for diabetes research.
UR - https://www.scopus.com/pages/publications/84937107193
UR - https://www.scopus.com/pages/publications/84937107193#tab=citedBy
U2 - 10.1007/s10529-014-1662-7
DO - 10.1007/s10529-014-1662-7
M3 - Article
C2 - 25257585
AN - SCOPUS:84937107193
SN - 0141-5492
VL - 37
SP - 227
EP - 233
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 1
ER -
