TY - JOUR
T1 - Genipin cross-linked chitosan–PVA composite films
T2 - An investigation on the impact of cross-linking on accelerating wound healing
AU - Panchal, Ruchira
AU - Mateti, Tarun
AU - Likhith, K.
AU - Rodrigues, Fiona Concy
AU - Thakur, Goutam
N1 - Funding Information:
The authors would like to acknowledge the Manipal Academy of Higher Education for providing the facility to conduct this research work. The authors also acknowledge Ms. Anubhuti Kashyap for her help in preparing the manuscript.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/9
Y1 - 2022/9
N2 - The applications of composite films in wound healing are immense, and the quest for suitable biomaterials drives research. Natural polymers lack mechanical strength; by cross-linking, their chemical nature can be changed through interlinking via intermolecular interactions rather than chemical bonds. This study explores the applications of cross-linked chitosan–polyvinyl alcohol–genipin films prepared using the solvent casting method. The cross-linked films were analyzed for their chemical structural changes, structural morphology, mechanical strength, and water retention capabilities. Comparative studies were performed with the un-crosslinked counterparts. Results indicated an increased tensile strength of 67.2% and improved water retention because of their compact structure. Further, Fourier-transform infrared spectroscopy confirmed that cross-linking occurred in the films, and scanning electron microscope micrographs showed that the films were uniform with a continuous morphology. Thereafter, an in vivo study was performed to assess the effectiveness of the films on wounds made on Albino Wistar rats. Histopathological analysis indicated quick fibroblast generation and angiogenesis using the cross-linked films, affirming their suitability for wound healing applications.
AB - The applications of composite films in wound healing are immense, and the quest for suitable biomaterials drives research. Natural polymers lack mechanical strength; by cross-linking, their chemical nature can be changed through interlinking via intermolecular interactions rather than chemical bonds. This study explores the applications of cross-linked chitosan–polyvinyl alcohol–genipin films prepared using the solvent casting method. The cross-linked films were analyzed for their chemical structural changes, structural morphology, mechanical strength, and water retention capabilities. Comparative studies were performed with the un-crosslinked counterparts. Results indicated an increased tensile strength of 67.2% and improved water retention because of their compact structure. Further, Fourier-transform infrared spectroscopy confirmed that cross-linking occurred in the films, and scanning electron microscope micrographs showed that the films were uniform with a continuous morphology. Thereafter, an in vivo study was performed to assess the effectiveness of the films on wounds made on Albino Wistar rats. Histopathological analysis indicated quick fibroblast generation and angiogenesis using the cross-linked films, affirming their suitability for wound healing applications.
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U2 - 10.1016/j.reactfunctpolym.2022.105339
DO - 10.1016/j.reactfunctpolym.2022.105339
M3 - Article
AN - SCOPUS:85134153456
SN - 1381-5148
VL - 178
JO - Reactive and Functional Polymers
JF - Reactive and Functional Polymers
M1 - 105339
ER -