Grape seed extract and zinc containing nutritional food supplement decreases the oxidative stress induced by carbon tetrachloride in rats

Objective: Current study was designed to investigate in vitro erythrocyte membrane stabilizing activity and in vivo antioxidant potential of combined formulation of grape seed extract and Zincovit tablets in carbon tetrachloride intoxicated rats. Methods: Erythrocyte membrane stabilizing activity was assessed by hypotonic solution-induced rabbit erythrocyte haemolysis. All the rats intoxicated with carbon tetrachloride were treated with combined formulation of grape seed extract and Zincovit tablets (40mg/kg, 80mg/kg and 160mg/kg; p.o) for 7 days except the toxic and positive control group. The extent of carbon tetrachloride induced oxidative stress was studied by enzymatic, non-enzymatic antioxidant parameters and liver histopathology in experimental rats. Results: Combined formulation of grape seed extract and Zincovit tablets showed 97.37% inhibition of hemolysis at the concentration of 1mg/ml. Rats treated with this test drug had significant antioxidant potential in comparison with toxic control group. The hepatic architecture of toxic control rats was found to acquire near-normalcy in Zincovit treated rats. Conclusions: Single combined formulation of grape seed extract and Zincovit tablets may be of great value in oxidative stress associated complications of human subjects as nutritional food supplement.