TY - JOUR
T1 - He-Ne laser irradiation in brinjal leads to metabolic rewiring and accumulation of bioactive components in fruits
AU - Swathy, Puthanvila Surendrababu
AU - Joshi, Manjunath B.
AU - Mahato, Krishna Kishore
AU - Muthusamy, Annamalai
N1 - Funding Information:
We thank Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India and TIFAC–CORE and FIST, DST, New Delhi, BUILDER Grant (BT/INF/22/SP43065/2021), DBT, New Delhi, and K-FIST, VGST, Govt. of Karnataka for the facilities. We are thankful to DAE-BRNS (Ref No.2012/34/47/BRNS) for the laser facility. The first author (SPS) is grateful to Manipal Academy of Higher Education (MAHE) for Dr. T.M.A. Pai PhD scholarship and International Cooperation Division, Department of Science and Technology (DST), Govt. of India, New Delhi for the Project Assistantship through the Indo-South Africa Joint Collaborative Project (DST/INT/South Africa/P-10/2016 dated 15 September 2016). We thank Prof. B.S. Satish Rao, Director, Manipal School of Life Sciences, MAHE for his constant encouragement and support. We would like to thank Mrs. Shashikala Tantry and Mr. Subhash Chandra for their experimental assistance. The authors are obliged to Prof. Radhakrishna Rao for his constant suggestions. The authors are also grateful to Mr. Mohan Rao and family for providing the field and maintenance of plants to carry out the study and Lakshmana Rao, Mr. Lakshman Mattu and Mr. Vasu Poojari, Mattu Village, Udupi District, Karnataka, India for the fruits and seeds. We would like to thank Mr. Sachin AT for his help in preparing the Fig. 5 and Ms. Arya K and for her help in the format of the manuscript. We are grateful to editor and reviewers for their comments to improve earlier version of the manuscript.
Funding Information:
We thank Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India and TIFAC CORE and FIST, DST, New Delhi, BUILDER Grant (BT/INF/22/SP43065/2021), DBT, New Delhi, and K-FIST, VGST, Govt. of Karnataka for the facilities. We are thankful to DAE-BRNS (Ref No.2012/34/47/BRNS) for the laser facility. The first author (SPS) is grateful to Manipal Academy of Higher Education (MAHE) for Dr. T.M.A. Pai PhD scholarship and International Cooperation Division, Department of Science and Technology (DST), Govt. of India, New Delhi for the Project Assistantship through the Indo-South Africa Joint Collaborative Project (DST/INT/South Africa/P-10/2016 dated 15 September 2016). We thank Prof. B.S. Satish Rao, Director, Manipal School of Life Sciences, MAHE for his constant encouragement and support. We would like to thank Mrs. Shashikala Tantry and Mr. Subhash Chandra for their experimental assistance. The authors are obliged to Prof. Radhakrishna Rao for his constant suggestions. The authors are also grateful to Mr. Mohan Rao and family for providing the field and maintenance of plants to carry out the study and Lakshmana Rao, Mr. Lakshman Mattu and Mr. Vasu Poojari, Mattu Village, Udupi District, Karnataka, India for the fruits and seeds. We would like to thank Mr. Sachin AT for his help in preparing the Fig. 5 and Ms. Arya K and for her help in the format of the manuscript. We are grateful to editor and reviewers for their comments to improve earlier version of the manuscript.
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Seed germination, seedling growth and healthy, virtue-based, uniform crop establishment are essential for considerable agricultural productivity. Physical and chemical techniques are widely utilized to promote and speed up seed germination. Due to their low cost, lack of side effects, environmental friendliness, non-destructive and low-power irradiation techniques such as laser, magnetic energy, and ultrasound are utilized successfully in various crops. The most well-known and well-studied laser in the agriculture sector is the Helium-Neon laser. The current study aimed to examine potential interactions between plants and laser rays and the possible use of the He-Ne laser to alter and enhance fruit metabolites. Fruits from field-grown brinjal (Solanum melongena L.) plants were collected at various developmental stages, such as 15, 30, 45, and 60 days post-anthesis (DPA), from the un-irradiated control and laser-irradiated plants for fruit metabolite profiling. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) indicated that the 15 DPA groups showed the highest variation compared to the other developmental stages. The observed metabolites were primarily divided into primary and secondary metabolites and are related to amino acids and derivatives, alkaloids and glycoalkaloids, fatty acids, organic acids, sugars, sugar alcohol, and terpenoids. Furthermore, the secondary metabolites were identified and classified into pigments, alkaloids, terpenoids, flavonoids, and glycoalkaloids. In the fruit tissues of the laser-irradiated groups, it was observed that the shikimate-derived phenolic pathway was not altered. On the other hand, the mevalonate route (MVP) and methylerythritol 4-phosphate (MEP) pathways were changed in the fruit tissues from the laser irradiation groups, according to the characterization of sterols, terpenoids, and alkaloids. Based on our previous reports and the current analysis, the biostimulatory role of the He-Ne laser was revealed with significant alterations in the abundance of bioactive molecules and their associated pathways in the developmental stages of brinjal fruits.
AB - Seed germination, seedling growth and healthy, virtue-based, uniform crop establishment are essential for considerable agricultural productivity. Physical and chemical techniques are widely utilized to promote and speed up seed germination. Due to their low cost, lack of side effects, environmental friendliness, non-destructive and low-power irradiation techniques such as laser, magnetic energy, and ultrasound are utilized successfully in various crops. The most well-known and well-studied laser in the agriculture sector is the Helium-Neon laser. The current study aimed to examine potential interactions between plants and laser rays and the possible use of the He-Ne laser to alter and enhance fruit metabolites. Fruits from field-grown brinjal (Solanum melongena L.) plants were collected at various developmental stages, such as 15, 30, 45, and 60 days post-anthesis (DPA), from the un-irradiated control and laser-irradiated plants for fruit metabolite profiling. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) indicated that the 15 DPA groups showed the highest variation compared to the other developmental stages. The observed metabolites were primarily divided into primary and secondary metabolites and are related to amino acids and derivatives, alkaloids and glycoalkaloids, fatty acids, organic acids, sugars, sugar alcohol, and terpenoids. Furthermore, the secondary metabolites were identified and classified into pigments, alkaloids, terpenoids, flavonoids, and glycoalkaloids. In the fruit tissues of the laser-irradiated groups, it was observed that the shikimate-derived phenolic pathway was not altered. On the other hand, the mevalonate route (MVP) and methylerythritol 4-phosphate (MEP) pathways were changed in the fruit tissues from the laser irradiation groups, according to the characterization of sterols, terpenoids, and alkaloids. Based on our previous reports and the current analysis, the biostimulatory role of the He-Ne laser was revealed with significant alterations in the abundance of bioactive molecules and their associated pathways in the developmental stages of brinjal fruits.
UR - https://www.scopus.com/pages/publications/85172444097
UR - https://www.scopus.com/pages/publications/85172444097#tab=citedBy
U2 - 10.1016/j.scienta.2023.112400
DO - 10.1016/j.scienta.2023.112400
M3 - Article
AN - SCOPUS:85172444097
SN - 0304-4238
VL - 322
JO - Scientia Horticulturae
JF - Scientia Horticulturae
M1 - 112400
ER -
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