TY - JOUR
T1 - High performance liquid chromatographic method for determination of clobetasol in rat plasma and its application to skin penetration
AU - Musmade, Prashant B.
AU - Deshpande, Praful B.
AU - Dengle, Swapnil J.
AU - Talole, Kranti B.
AU - Pathak, Shriram M.
AU - Adiga, M. N.S.
AU - Bhat, Krishnamurthy
AU - Udupa, Nayanabhirama
AU - Laxminarayana Bairy, K.
PY - 2010/2
Y1 - 2010/2
N2 - A simple and sensitive high performance liquid chromatography (HPLC) method was developed for quantification of clobetasol (CLB) in rat plasma. Aripiprazole was used as an internal standard (IS). The present method uses protein precipitation with acetonitrile, followed by the liquid-liquid extraction with methyl tertiary butyl ether for extraction of the CLB from the matrix. Separation was carried out using Variance C18 (250.0 × 4.6 mm, 5 μm particle size) column and the effluent was monitored by an ultra violet (UV) detector at 240 nm. The mobile phase used was acetonitrile: phosphate buffer (pH 7.0; 25.0mM containing 0.2 %v/v triethylamine) (65: 35 % v/v) at a flow rate of 1.0 ml/min. The present method was validated as per the United States Food and Drug Administration (USFDA) guideline. This method was linear over the range of 25.0-1000.0 ng/ml with regression coefficient greater than 0.99. The mean recovery of CLB and IS were 70.8±1.9 and 83.32±1.55 % respectively. The method was found to be precise, accurate and specific. The developed HPLC method was applied for the estimation of CLB in plasma after topical application of various zinc salts in rats to study the penetration of CLB through rat skin.
AB - A simple and sensitive high performance liquid chromatography (HPLC) method was developed for quantification of clobetasol (CLB) in rat plasma. Aripiprazole was used as an internal standard (IS). The present method uses protein precipitation with acetonitrile, followed by the liquid-liquid extraction with methyl tertiary butyl ether for extraction of the CLB from the matrix. Separation was carried out using Variance C18 (250.0 × 4.6 mm, 5 μm particle size) column and the effluent was monitored by an ultra violet (UV) detector at 240 nm. The mobile phase used was acetonitrile: phosphate buffer (pH 7.0; 25.0mM containing 0.2 %v/v triethylamine) (65: 35 % v/v) at a flow rate of 1.0 ml/min. The present method was validated as per the United States Food and Drug Administration (USFDA) guideline. This method was linear over the range of 25.0-1000.0 ng/ml with regression coefficient greater than 0.99. The mean recovery of CLB and IS were 70.8±1.9 and 83.32±1.55 % respectively. The method was found to be precise, accurate and specific. The developed HPLC method was applied for the estimation of CLB in plasma after topical application of various zinc salts in rats to study the penetration of CLB through rat skin.
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U2 - 10.4172/1948-593X.1000013
DO - 10.4172/1948-593X.1000013
M3 - Article
AN - SCOPUS:79952607422
SN - 1948-593X
VL - 2
SP - 1
EP - 7
JO - Journal of Bioanalysis and Biomedicine
JF - Journal of Bioanalysis and Biomedicine
IS - 1
ER -