TY - JOUR
T1 - High-performance liquid chromatography and pharmacokinetics of aceclofenac in rats
AU - Musmade, P.
AU - Subramanian, G.
AU - Srinivasan, K.K.
N1 - Cited By :37
Export Date: 10 November 2017
CODEN: ACACA
Correspondence Address: Musmade, P.; Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal, 576104, India; email: prashant.pqa@manipal.edu
Chemicals/CAS: aceclofenac, 89796-99-6; ibuprofen, 15687-27-1; aceclofenac, 89796-99-6; Anti-Inflammatory Agents, Non-Steroidal; Diclofenac, 15307-86-5; Ethylamines; Ibuprofen, 15687-27-1; Methanol, 67-56-1; triethylamine, 121-44-8
Manufacturers: Karnataka, India; Sun, India
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PY - 2007
Y1 - 2007
N2 - A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for quantification of aceclofenac in rat plasma. Ibuprofen was used as an internal standard (IS). The present method used protein precipitation for extraction of aceclofenac from rat plasma. Separation was carried out on reversed-phase C18 column (250 mm × 4.6 mm, 5 μ) and the column effluent was monitored by UV detector at 282 nm. The mobile phase used was methanol-triethylamine (pH 7.0; 0.3% v/v in Milli-Q water) (60:40%, v/v) at a flow rate of 1.0 mL min-1. This method was linear over the range of 50.0-3500.0 ng mL-1 with regression coefficient greater than 0.99. The mean recovery of aceclofenac and IS were 84.62 ± 3.23 and 89.19 ± 1.57%, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of aceclofenac in rats. © 2006 Elsevier B.V. All rights reserved.
AB - A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for quantification of aceclofenac in rat plasma. Ibuprofen was used as an internal standard (IS). The present method used protein precipitation for extraction of aceclofenac from rat plasma. Separation was carried out on reversed-phase C18 column (250 mm × 4.6 mm, 5 μ) and the column effluent was monitored by UV detector at 282 nm. The mobile phase used was methanol-triethylamine (pH 7.0; 0.3% v/v in Milli-Q water) (60:40%, v/v) at a flow rate of 1.0 mL min-1. This method was linear over the range of 50.0-3500.0 ng mL-1 with regression coefficient greater than 0.99. The mean recovery of aceclofenac and IS were 84.62 ± 3.23 and 89.19 ± 1.57%, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of aceclofenac in rats. © 2006 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.aca.2006.11.080
DO - 10.1016/j.aca.2006.11.080
M3 - Article
SN - 0003-2670
VL - 585
SP - 103
EP - 109
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -