TY - JOUR
T1 - High-sensitivity profiling of glycoproteins from ovarian cancer sera using lectin-affinity and LC-ESI-Q-TOF-MS/MS
AU - Roopashri, Arekal N.
AU - Divyashree, M. S.
AU - Savitha, J.
N1 - Funding Information:
The authors wish to gratefully acknowledge University Grants Commission (UGC) – Dr. D.S. Kothari Postdoctoral fellowship scheme (No.F.4-2/2006(BSR)/BL/18-19/0159), India, for providing financial support to carry out this research work and Bangalore University for allowing us to utilize the laboratory facilities at the Department of Microbiology, Biotechnology and Food Technology, Bengaluru, India. They wish to acknowledge the Indian Institute of Science (IISC), Bengaluru, India, for having carried out the LC-ESI-Q-TOF-MS/MS analysis.
Funding Information:
The authors wish to gratefully acknowledge University Grants Commission (UGC) – Dr. D.S. Kothari Postdoctoral fellowship scheme (No.F.4-2/2006(BSR)/BL/18-19/0159), India, for providing financial support to carry out this research work and Bangalore University for allowing us to utilize the laboratory facilities at the Department of Microbiology, Biotechnology and Food Technology, Bengaluru, India. They wish to acknowledge the Indian Institute of Science (IISC), Bengaluru, India, for having carried out the LC-ESI-Q-TOF-MS/MS analysis.
Publisher Copyright:
© 2023 The Authors
PY - 2023/1
Y1 - 2023/1
N2 - Lectins act as effective diagnostic tools for screening potential cancer biomarkers because of their ability to recognize the carbohydrate moiety of glycoproteins present in most complex tissues and fluids. This study aimed to propose an ideal procedure for the precise recovery of glycoprotein-derived oligosaccharides using freshwater microalgal lectins and to validate their effectiveness in the enrichment of glycoproteins present in the sera. A total of 5 ovarian cancer (OC) patients with grade III were included in the present study to identify potential biomarkers present in the serum samples. Proteomic analysis of OC serum samples was carried out using lectin-affinity entrapment followed by affinity chromatography coupled with liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-Q-TOF-MS/MS) methods. Proteins with the highest Mascot score were found to be expressed in the OC III serum group and were identified as serotransferrin (TRF), haptoglobin, hemopexin, serine protease 1 (Sp1), ceruloplasmin, alpha-1-antitrypsin, alpha-1-antichymotrypsin (ACT), alpha-2-HS glycoprotein (AHSG), and immunoglobulins (Igs). Among them serine protease 1 was recovered at a high-level using lectin-affinity techniques. PRSS 1 gene is responsible for the expression of Sp1 in OC serum. The present study evaluated the usefulness of microalgal lectins in the identification of molecular markers present in serum samples of OC. This data provide novel insights regarding the presence of Sp1 in OC serum specimen, paving the way for further investigations related to glycosylation patterns of Sp1 and clinical applicability.
AB - Lectins act as effective diagnostic tools for screening potential cancer biomarkers because of their ability to recognize the carbohydrate moiety of glycoproteins present in most complex tissues and fluids. This study aimed to propose an ideal procedure for the precise recovery of glycoprotein-derived oligosaccharides using freshwater microalgal lectins and to validate their effectiveness in the enrichment of glycoproteins present in the sera. A total of 5 ovarian cancer (OC) patients with grade III were included in the present study to identify potential biomarkers present in the serum samples. Proteomic analysis of OC serum samples was carried out using lectin-affinity entrapment followed by affinity chromatography coupled with liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-Q-TOF-MS/MS) methods. Proteins with the highest Mascot score were found to be expressed in the OC III serum group and were identified as serotransferrin (TRF), haptoglobin, hemopexin, serine protease 1 (Sp1), ceruloplasmin, alpha-1-antitrypsin, alpha-1-antichymotrypsin (ACT), alpha-2-HS glycoprotein (AHSG), and immunoglobulins (Igs). Among them serine protease 1 was recovered at a high-level using lectin-affinity techniques. PRSS 1 gene is responsible for the expression of Sp1 in OC serum. The present study evaluated the usefulness of microalgal lectins in the identification of molecular markers present in serum samples of OC. This data provide novel insights regarding the presence of Sp1 in OC serum specimen, paving the way for further investigations related to glycosylation patterns of Sp1 and clinical applicability.
UR - https://www.scopus.com/pages/publications/85149827677
UR - https://www.scopus.com/pages/publications/85149827677#tab=citedBy
U2 - 10.1016/j.crbiot.2023.100122
DO - 10.1016/j.crbiot.2023.100122
M3 - Article
AN - SCOPUS:85149827677
SN - 2590-2628
VL - 5
JO - Current Research in Biotechnology
JF - Current Research in Biotechnology
M1 - 100122
ER -
SDG 3 Good Health and Well-being