TY - JOUR
T1 - HSV susceptibility to acyclovir – genotypic and phenotypic characterization
AU - Vikas, Raksha
AU - Prabhu, Suresha G.
AU - Mudgal, Piya P.
AU - Shetty, Ujwal
AU - Karunakaran, Kavitha
AU - Jagadesh, Anitha
AU - Auti, Amogh
AU - Stansilaus, Rithu P.
AU - Nair, Sudheesh
AU - Arunkumar, Govindakarnavar
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Background: Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples. Methods: A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique. Results: Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay. Conclusions: These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.
AB - Background: Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples. Methods: A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique. Results: Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay. Conclusions: These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.
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U2 - 10.3851/IMP3279
DO - 10.3851/IMP3279
M3 - Article
C2 - 30507553
AN - SCOPUS:85065894334
SN - 1359-6535
VL - 24
SP - 141
EP - 145
JO - Antiviral Therapy
JF - Antiviral Therapy
IS - 2
ER -
