HSV susceptibility to acyclovir – genotypic and phenotypic characterization

Raksha Vikas; Suresha G. Prabhu; Piya P. Mudgal; Ujwal Shetty; Kavitha Karunakaran; Anitha Jagadesh; Amogh Auti; Rithu P. Stansilaus; Sudheesh Nair; Govindakarnavar Arunkumar

doi:10.3851/IMP3279

HSV susceptibility to acyclovir – genotypic and phenotypic characterization

Raksha Vikas
, Suresha G. Prabhu
, Piya P. Mudgal
, Ujwal Shetty
, Kavitha Karunakaran
, Anitha Jagadesh
, Amogh Auti
, Rithu P. Stansilaus
, Sudheesh Nair
, Govindakarnavar Arunkumar^*

^*Corresponding author for this work

Manipal Institute of Virology, Manipal

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Background: Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples. Methods: A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique. Results: Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay. Conclusions: These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.

Original language	English
Pages (from-to)	141-145
Number of pages	5
Journal	Antiviral Therapy
Volume	24
Issue number	2
DOIs	https://doi.org/10.3851/IMP3279
Publication status	Published - 01-01-2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmacology (medical)
Infectious Diseases

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10.3851/IMP3279

Cite this

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title = "HSV susceptibility to acyclovir – genotypic and phenotypic characterization",

abstract = "Background: Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples. Methods: A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique. Results: Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay. Conclusions: These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.",

author = "Raksha Vikas and Prabhu, \{Suresha G.\} and Mudgal, \{Piya P.\} and Ujwal Shetty and Kavitha Karunakaran and Anitha Jagadesh and Amogh Auti and Stansilaus, \{Rithu P.\} and Sudheesh Nair and Govindakarnavar Arunkumar",

note = "Publisher Copyright: {\textcopyright} 2019 International Medical Press",

year = "2019",

month = jan,

day = "1",

doi = "10.3851/IMP3279",

language = "English",

volume = "24",

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journal = "Antiviral Therapy",

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TY - JOUR

T1 - HSV susceptibility to acyclovir – genotypic and phenotypic characterization

AU - Vikas, Raksha

AU - Prabhu, Suresha G.

AU - Mudgal, Piya P.

AU - Shetty, Ujwal

AU - Karunakaran, Kavitha

AU - Jagadesh, Anitha

AU - Auti, Amogh

AU - Stansilaus, Rithu P.

AU - Nair, Sudheesh

AU - Arunkumar, Govindakarnavar

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples. Methods: A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique. Results: Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay. Conclusions: These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.

AB - Background: Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples. Methods: A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique. Results: Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay. Conclusions: These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.

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UR - https://www.scopus.com/pages/publications/85065894334#tab=citedBy

U2 - 10.3851/IMP3279

DO - 10.3851/IMP3279

M3 - Article

C2 - 30507553

AN - SCOPUS:85065894334

SN - 1359-6535

VL - 24

SP - 141

EP - 145

JO - Antiviral Therapy

JF - Antiviral Therapy

IS - 2

ER -

HSV susceptibility to acyclovir – genotypic and phenotypic characterization

Abstract

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