Human Mesenchymal Stromal Cells-Derived Conditioned Medium Based Formulation For Advanced Skin Care: In Vitro And In Vivo Evaluation

Photo aging of the skin is primarily due to alterations in the dermal extracellular matrix. Apart from physiological aging, some environmental factors accelerate the skin aging and are characterized by wrinkles, laxity, roughness and irregular pigmentation. Mesenchymal stromal cell derived growth factors and cytokines are used in cosmetic products because of their ability to repair and regenerate the damaged skin. In the present study, Conditioned Medium (CM) derived from human mesenchymal stromal cells was produced, formulated and the CM based formulation was evaluated for anti-aging potential and skin rejuvenation. The particle size analysis and HPLC analysis revealed that the proteins were dispersed in a nano-size range in the concentrated conditioned medium. The total protein content analysis and bioassay results showed that the proteins were stable in the pH range of 5.0 to 8.0. The in vitro scratch wound assay and cyclobutane pyrimidine dimer formation assay of the CM based formulation promoted the migration of cells and resulted in significant decrease in the formation of CPD respectively when compared to untreated cells. The histological examination of nude mice sections revealed that treatment with CM based formulation reduced the extent of hyperkeratosis, epidermal hyperplasia and inflammatory cells in the dermis, improved skin moisture level and showed lesser collagen degradation when compared to animals exposed to UVB irradiation. These results suggest the photo protective effects of CM based formulation and its potential in reducing the UV induced skin damage thereby promoting the regeneration of aging skin.