TY - JOUR
T1 - Human tear fluid analysis for clinical applications
T2 - progress and prospects
AU - Adigal, Sphurti S.
AU - Rizvi, Alisha
AU - Rayaroth, Nidheesh V.
AU - John, Reena V.
AU - Barik, Ajayakumar
AU - Bhandari, Sulatha
AU - George, Sajan D.
AU - Lukose, Jijo
AU - Kartha, Vasudevan B.
AU - Chidangil, Santhosh
N1 - Funding Information:
Authors are thankful to VGST (GRD No. 459), Govt. of Karnataka, for the sanction of a grant to establish an HPLC facility under the scheme Centre of Excellence in Science, Engineering and Medicine (CESEM) and DST-FIST for supporting the department to setup a nano bioimaging facility. Ms. Sphurti Adigal is thankful to Manipal Academy of Higher Education for the Dr. TMA Pai Ph.D. Scholarship.
Publisher Copyright:
© 2021 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - Introduction: Human blood and saliva are increasingly under investigation for the detection of biomarkers for early diagnosis of non-communicable (e.g.cancers) and communicable diseases like COVID-19. Exploring the potential application of human tears, an easily accessible body fluid, for the diagnosis of various diseases is the need of the hour. Areas covered: This review deals with a comprehensive account of applications of tear analysis using different techniques, their comparison and overall progress achieved till now. The techniques used for tear fluid analysis are HPLC/UPLC/SDS-PAGE, CE, etc., together with ELISA, Mass Spectrometry, etc. But, with advances in instrumentation and data processing methods, it has become easy to couple the various separation methods with highly sensitive optical techniques for the analysis of body fluids. Expert opinion: Tear analysis can provide valuable information about the health condition of the eyes since it contains several molecular constituents, and their relative concentrations may alter under abnormal conditions. Tear analysis has the advantage that it is totally non-invasive. This study recommends tear fluid as a reliable clinical sample to be probed by highly sensitive optical techniques to diagnose different health conditions, with special emphasis on eye diseases.
AB - Introduction: Human blood and saliva are increasingly under investigation for the detection of biomarkers for early diagnosis of non-communicable (e.g.cancers) and communicable diseases like COVID-19. Exploring the potential application of human tears, an easily accessible body fluid, for the diagnosis of various diseases is the need of the hour. Areas covered: This review deals with a comprehensive account of applications of tear analysis using different techniques, their comparison and overall progress achieved till now. The techniques used for tear fluid analysis are HPLC/UPLC/SDS-PAGE, CE, etc., together with ELISA, Mass Spectrometry, etc. But, with advances in instrumentation and data processing methods, it has become easy to couple the various separation methods with highly sensitive optical techniques for the analysis of body fluids. Expert opinion: Tear analysis can provide valuable information about the health condition of the eyes since it contains several molecular constituents, and their relative concentrations may alter under abnormal conditions. Tear analysis has the advantage that it is totally non-invasive. This study recommends tear fluid as a reliable clinical sample to be probed by highly sensitive optical techniques to diagnose different health conditions, with special emphasis on eye diseases.
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U2 - 10.1080/14737159.2021.1941879
DO - 10.1080/14737159.2021.1941879
M3 - Review article
C2 - 34115952
AN - SCOPUS:85108331508
SN - 1473-7159
VL - 21
SP - 767
EP - 787
JO - Expert Review of Molecular Diagnostics
JF - Expert Review of Molecular Diagnostics
IS - 8
ER -
