Hydrolysis of rice bran oil using immobilized lipase in a stirred batch reactor

V. Ramachandra Murty, Jayadev Bhat, P. K.A. Muniswaran

Research output: Contribution to journalArticlepeer-review

11 Citations (SciVal)


Candida cylindracea lipase was immobilized by adsorption on acid washed glass beads. It was observed that protein loading of the support depends on the size of the particle, with smaller particle containing higher amount of protein per unit weight. Initial reaction rate linearly varied up to enzyme concentration of 17.25 U/mL. Amount of free fatty acids produced was linearly proportional up to the enzyme loading of 1650 μg/g of bead. Achievement of chemical equilibrium took longer time in the case of less protein loading. Degree of hydrolysis was found to decrease in second and third consecutive batch operations on repeated use of immobilized lipase.

Original languageEnglish
Pages (from-to)367-370
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Issue number6
Publication statusPublished - 01-01-2002

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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