TY - JOUR
T1 - Identification and determination of Protocatechuic acid present in Greater Cardamom fruit extracts by HPTLC technique
AU - Shivanand, P.
AU - Mahalaxmi, R.
N1 - Cited By :1
Export Date: 10 November 2017
Correspondence Address: Shivanand, P.; MCOPS, Manipal, Manipal University, Udupi, Karnataka, India; email: [email protected]
Chemicals/CAS: acetone, 67-64-1; chloroform, 67-66-3; methanol, 67-56-1; petroleum ether, 8032-32-4; protocatechuic acid, 99-50-3
Manufacturers: LGC Promochem, India
References: Rajani, M., Kanaki, N.S., (2008) Phytochemical standardization of herbal drugs and polyherbal formulations, , In: Bioactive Molecules and Medicinal Plants. Editors; (1999) The Ayurvedic Pharmacopoeia of India, 2, pp. 158-159. , Anonymous, Govt. of India, First edition, Part I; (2006) A dictionary of Indian Raw Materials and Industrial research, 1, pp. 226-229. , Wealth of India, Revised Edition Reprinted; Joshi, S.C., Joshi, V., Effect of Amomum subulatum on oxidative stress and atherosclerosis in cholesterol fed rabbits (2007) Pharmacologyonline, 1, pp. 451-463. , http://www.unisa.it/download/1966_10305_1269820156_47_Joshi.pdf; Ravichandran, G., Bharadwaj, V.S., Kolhapure, S.A., Evaluation of the efficacy and safety of "Anti- Wrinkle cream" in the treatment of facial skin wrinkles: A prospective, open, phase III clinical trial (2005) The Antiseptic, 102 (2), pp. 65-70; Validation of Analytical Procedure: Methodology (1994) Proc. Int. Con. Harmonization, , ICH, Q2A, In, Geneva; Yadav, A.S., Bhatnagar, D., Modulatory effect of spice extracts on iron-induced lipid peroxidation in rat liver (2007) Biofactors, 29 (2-3), pp. 147-157; Ramawat, K.G., Merillon, J.M., , pp. 349-369. , Springer-Verlag, Berlin Heidelberg, 978-3-540-74603-4; Evans, W.C., (2002) Trease and Evans Pharmacognosy, pp. 513-525. , W B Saunders, London, 15th edn, 978-81- 312-0087-2; Harborne, J.B., (2005) Phytochemical Method - A guide to modern techniques of plant analysis, pp. 43-45. , Springer, 3rd edn, 978-81-8128-310-8; Kikuzaki, H., Kawai, Y., Nakatani, N., 1, 1- Diphenyl-2-picrylhydrazyl radical-scavenging active compounds from greated cardamom (Amomum subulatum Roxb.) (2001) J Nutr Sci Vitaminol, 47 (2), pp. 167-171. , (Tokyo)
PY - 2010
Y1 - 2010
N2 - The aim of present study was development of simple, rapid and accurate HPTLC method for estimation of Protocatechuic acid in various extracts of Amumum subulatum Roxb. fruit constituents (Family Zingiberacea), commonly known as 'Badi Elaichi' or Greater Cardamom. The powdered drug was subjected to extraction by soxhlet apparatus using methanol and acetone separately as well as petroleum ether (40-60), chloroform, methanol and water successively. The extracts were screened for presence of various phytoconstituents using preliminary chemical tests. Protocatechuic acid was estimated in methanol and acetone extract by HPTLC method. Detection and Quantification was performed at wavelength 254nm. The Acetone and methanol extracts were found to contain 1.04846 and 0.8634 %w/w Protocatechuic acid respectively by using validated method. Since this method resolves and quantifies protocatechuic acid accurately and precisely, it can be useful for quantification of the compound in herbal formulation.
AB - The aim of present study was development of simple, rapid and accurate HPTLC method for estimation of Protocatechuic acid in various extracts of Amumum subulatum Roxb. fruit constituents (Family Zingiberacea), commonly known as 'Badi Elaichi' or Greater Cardamom. The powdered drug was subjected to extraction by soxhlet apparatus using methanol and acetone separately as well as petroleum ether (40-60), chloroform, methanol and water successively. The extracts were screened for presence of various phytoconstituents using preliminary chemical tests. Protocatechuic acid was estimated in methanol and acetone extract by HPTLC method. Detection and Quantification was performed at wavelength 254nm. The Acetone and methanol extracts were found to contain 1.04846 and 0.8634 %w/w Protocatechuic acid respectively by using validated method. Since this method resolves and quantifies protocatechuic acid accurately and precisely, it can be useful for quantification of the compound in herbal formulation.
M3 - Article
SN - 0976-044X
VL - 1
SP - 27
EP - 32
JO - International Journal of Pharmaceutical Sciences Review and Research
JF - International Journal of Pharmaceutical Sciences Review and Research
IS - 1
ER -