Immobilisation of cyclodextrin glucanotransferase from Bacillus circulans ATCC 21783 on purified seasand

Jyoti L. Iyer; Premalatha Shetty; Jagadish S. Pai

doi:10.1007/s10295-002-0009-x

Immobilisation of cyclodextrin glucanotransferase from Bacillus circulans ATCC 21783 on purified seasand

Jyoti L. Iyer, Premalatha Shetty, Jagadish S. Pai^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Cyclodextrin glucanotransferase (CGTase) from Bacillus circulans (ATCC 21783) was immobilised on a silica-based support: purified seasand. Although adsorption of 98% was achieved, considerable desorption was encountered. This problem was minimised by crosslinking the adsorbed enzyme with glutaraldehyde. The immobilised enzyme after crosslinking could be used repeatedly for cyclodextrin (CD) production in a batch process. The activity retention was 80% at the end of the eighth cycle. The immobilised enzyme showed a shift in the pH optimum towards the alkaline side and also an improvement in the pH stability compared to the free enzyme. It catalysed the formation of β-CD as a major product. A significant amount of α-CD production was also observed on prolonged incubation.

Original language	English
Pages (from-to)	47-51
Number of pages	5
Journal	Journal of Industrial Microbiology and Biotechnology
Volume	30
Issue number	1
DOIs	https://doi.org/10.1007/s10295-002-0009-x
Publication status	Published - 01-01-2003

All Science Journal Classification (ASJC) codes

General Medicine

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title = "Immobilisation of cyclodextrin glucanotransferase from Bacillus circulans ATCC 21783 on purified seasand",

abstract = "Cyclodextrin glucanotransferase (CGTase) from Bacillus circulans (ATCC 21783) was immobilised on a silica-based support: purified seasand. Although adsorption of 98\% was achieved, considerable desorption was encountered. This problem was minimised by crosslinking the adsorbed enzyme with glutaraldehyde. The immobilised enzyme after crosslinking could be used repeatedly for cyclodextrin (CD) production in a batch process. The activity retention was 80\% at the end of the eighth cycle. The immobilised enzyme showed a shift in the pH optimum towards the alkaline side and also an improvement in the pH stability compared to the free enzyme. It catalysed the formation of β-CD as a major product. A significant amount of α-CD production was also observed on prolonged incubation.",

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AU - Iyer, Jyoti L.

AU - Shetty, Premalatha

AU - Pai, Jagadish S.

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AB - Cyclodextrin glucanotransferase (CGTase) from Bacillus circulans (ATCC 21783) was immobilised on a silica-based support: purified seasand. Although adsorption of 98% was achieved, considerable desorption was encountered. This problem was minimised by crosslinking the adsorbed enzyme with glutaraldehyde. The immobilised enzyme after crosslinking could be used repeatedly for cyclodextrin (CD) production in a batch process. The activity retention was 80% at the end of the eighth cycle. The immobilised enzyme showed a shift in the pH optimum towards the alkaline side and also an improvement in the pH stability compared to the free enzyme. It catalysed the formation of β-CD as a major product. A significant amount of α-CD production was also observed on prolonged incubation.

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Immobilisation of cyclodextrin glucanotransferase from Bacillus circulans ATCC 21783 on purified seasand

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