TY - JOUR
T1 - Impact of prepubertal bovine ovarian tissue pre-freeze holding duration on follicle quality
AU - Amonkar, Divya Deeleep Bhagat
AU - Genovese, Vincenzo
AU - De Gregorio, Vincenza
AU - Travaglione, Angela
AU - Uppangala, Shubhashree
AU - Vani Lakshmi, R.
AU - Kalthur, Guruprasad
AU - Gualtieri, Roberto
AU - Talevi, Riccardo
AU - Adiga, Satish Kumar
N1 - Funding Information:
DDBA acknowledge the Dr. TMA Pai PhD Fellowship from the Manipal Academy of Higher Education (MAHE).
Funding Information:
This study was partly supported by Indian Council for Medical Research (Grant# 5/10/FR/52/2020-RBMCH ).
Publisher Copyright:
© 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
PY - 2023/9
Y1 - 2023/9
N2 - Ovarian tissue cryopreservation prior to gonadotoxic treatment is the only recommended option for fertility preservation in prepubertal girls. Due to the technical complexity of this technique, limited number of centres across the world are equipped to offer the facility. Hence, the retrieved ovarian tissue needs to be maintained at hypothermic temperature (4 °C) for long time during shipment. The time taken between tissue retrieval and cryopreservation could influence the functionality of cells during fertility restoration. This study explored the tissue integrity and follicle quality of ovarian cortical slices subjected to pre-freeze holding for various time durations in vitro. Prepubertal bovine ovarian tissue from < 12 months old animals were handled at hypothermic holding (4 °C) for 0, 24, 48 and 72 h. The tissues were assessed for follicle viability through confocal analysis of live-dead labelled samples, and follicle quality and tissue integrity through histology. Results have shown that follicle viability, and overall follicle quality were not significantly affected at the end of 72 h hypothermic holding. Though, the observation reassures extended hypothermic holding prior to freezing, findings need to be validated in human tissue prior to use in clinical fertility preservation programs.
AB - Ovarian tissue cryopreservation prior to gonadotoxic treatment is the only recommended option for fertility preservation in prepubertal girls. Due to the technical complexity of this technique, limited number of centres across the world are equipped to offer the facility. Hence, the retrieved ovarian tissue needs to be maintained at hypothermic temperature (4 °C) for long time during shipment. The time taken between tissue retrieval and cryopreservation could influence the functionality of cells during fertility restoration. This study explored the tissue integrity and follicle quality of ovarian cortical slices subjected to pre-freeze holding for various time durations in vitro. Prepubertal bovine ovarian tissue from < 12 months old animals were handled at hypothermic holding (4 °C) for 0, 24, 48 and 72 h. The tissues were assessed for follicle viability through confocal analysis of live-dead labelled samples, and follicle quality and tissue integrity through histology. Results have shown that follicle viability, and overall follicle quality were not significantly affected at the end of 72 h hypothermic holding. Though, the observation reassures extended hypothermic holding prior to freezing, findings need to be validated in human tissue prior to use in clinical fertility preservation programs.
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U2 - 10.1016/j.repbio.2023.100794
DO - 10.1016/j.repbio.2023.100794
M3 - Article
AN - SCOPUS:85166308563
SN - 1642-431X
VL - 23
JO - Reproductive biology
JF - Reproductive biology
IS - 3
M1 - 100794
ER -