Objective: The aim of this study was to evaluate the antimicrobial efficacy of 7% maleic acid (MA) and 17% ethylenediaminetetraacetic acid (EDTA) in elimination of Enterococcus faecalis, Candida albicans, and Staphylococcus aureus at different time intervals. Study design: Transfer culture of microbial strains were used for inoculum preparation and determination of time-kill assay. The viability counts of 7% MA and 17% EDTA suspensions were performed at 0, 2, 4, 6, 12, and 24 hours. Assay results were analyzed by determining number of strains that yielded log 10 CFU/mL of -1 compared with counts at 0 hours, for test medicaments at time intervals. Medicaments were considered to be microbicidal at a minimum inhibitory concentration that reduced original inoculum by >3 log 10 CFU/mL (99.9%) and microbiostatic if inoculum was reduced by <3 log 10 CFU/mL. Statistical analysis was performed using chi-square and Fisher exact tests as well as Friedman test for comparison of the time interval within the MA and EDTA groups. Results: At all time intervals, there was no significant difference between MA and EDTA for all of the organisms (P >.05). However, within the MA and EDTA groups at various time intervals, there were significant differences (P <.001). Conclusions: Equivalent antimicrobial activity was observed by MA and EDTA against all of the organisms tested at various periods.

Original languageEnglish
Pages (from-to)696-700
Number of pages5
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Issue number5
Publication statusPublished - 01-11-2011

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • General Dentistry


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