In vitro antioxidant studies on the benzyl tetra isoquinoline alkaloid berberine

A. Shirwaikar, K. Rajendran, I.S.R. Punitha

Research output: Contribution to journalArticlepeer-review

296 Citations (Scopus)

Abstract

Berberine is a benzyl tetra isoquinoline alkaloid which is widely used as an antimicrobial and an antidiarrhoeal. As berberine containing plants are virtually used in all forms of traditional medicine, our study aimed to examine the antioxidant activity of berberine using 2,2-diphenyl 1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide scavenging, lipid peroxidation, superoxide scavenging, iron chelating activity and 2,2-azino bis(3-ethylbenzothiazoline-6- sulfonate) (ABTS) radical scavenging methods. The IC50 values for all the models were calculated by regression analysis. In all the models tested, berberine showed its ability to scavenge the free radicals in a concentration dependent manner. The present study thereby justifies the therapeutic potential of berberine. © 2006 Pharmaceutical Society of Japan.
Original languageEnglish
Pages (from-to)1906-1910
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number9
DOIs
Publication statusPublished - 2006

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