In vitro assessment of total phenolic content, Iron chelating, reducing potential and dpph scavenging activity of cynodon dactylon hydroalcoholic extract

Cynodon dactylon is generally known as Druva grass exhibiting various medicinal properties and used in folk medicine. The current study we examined antioxidant properties along with estimation of total phenolic content, reducing property, Iron chelating capacity of hydro alcoholic extract Cynodon dactylon Modified method of Folin-Ciocalteu was used for Total phenolic content estimation of the plant extract. The capacity of Iron chelating by Cynodon datylon extract was studied by slightly customized procedure explained by Dinis. The ability of scavenging 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical was calculated in percentage by a reduction of absorbance at 517 nm as described by Hou comparing with standard Ascorbic acid and Ferric reducing antioxidant potential assay method described by Benzie and Strain. Cynodon dactylon displayed phenolic content ranging from 30 to140 mg GAE (Gallic acid equivalent)/g of extract. At 10 µg/ml concentration hydroalcoholic entire plant extract of Cynodon dactylon displayed 60.4 ±0.9% Iron chelating capacity when compared to standard 89.77±0.33%. The maximum reducing ability (absorbance 0.729±0.44) of plant extract showed at 1mg/ml and the standard Rutin showed (absorbance 2.983±0.55) at same concentration. The maximum scavenging ability (87.2%) of plant extract showed at 1mg/ml and the standard Ascorbic acid showed saturation at high concentration. The entire plant extract of Cynodon dactylon showed its scavenging capacity of free radicals in a concentration dependant method. The results of this studies shows that the hydro alcoholic extract of entire Cynodon dactylon plant is affluent in antioxidant components which might afford scientific basis for its use in phyto-medicine.