In vitro effect of proteases on human and rat plasma α-macroglobulin activity

R. S. Kumar, T. N. Pattabiraman

Research output: Contribution to journalArticlepeer-review


Human plasma α2- macroglobulin activity decreased upon incubation for 12 hours at 37°C in the presence of aminopeptidase (5 μg/0.1 ml), while the immunoreactivity of the inhibitor did not significantly alter under the same conditions. This observation indicates that the inhibitor protein was not extensively degraded on treatment with aminopeptidase. Inactivation of α2 - macroglobulin by aminopeptidase was found to be a pH and temperature dependent process. While Streptomyces caesiptosus protease (5 μg/0.05 ml) inactivated partially purified human plasma α2-macroglobulin within fifteen minutes of incubation at 37°C, the action of Streptomyces griseus (15 μg), pronase (15 μg) and aminopeptidase (5μg) appeared to be time dependent. The α-macroglobulin activity in ten fold diluted rat plasma was completely abolished in the presence of Bacillus amyloliquifaciens protease (15μg) whereas, moderate inactivation was observed in the presence of Streptomyces griseus protease (15 μg) and Streptomyces caesiptosus protease (15 μg), upon incubation at 37°C for 12 hours.

Original languageEnglish
Pages (from-to)127-135
Number of pages9
JournalBiochemical Archives
Issue number3
Publication statusPublished - 01-01-1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry


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