TY - JOUR
T1 - In vitro free radical scavenging potential of methanol extract of entire plant of Phyllanthus reticulatus poir
AU - Aswatha Ram, H.N.
AU - Shreedhara, C.S.
AU - Gajera, F.P.
AU - Zanwar, S.B.
N1 - Cited By :4
Export Date: 10 November 2017
Correspondence Address: Aswatha Ram, H.N.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal - 576 104 Karnataka, India; email: aswatharam@google.com
Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; hemoglobin, 9008-02-0; lipid, 66455-18-3; methanol, 67-56-1; nitric oxide, 10102-43-9; phenanthroline, 12678-01-2; superoxide, 11062-77-4; tocopherol, 1406-66-2
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PY - 2008
Y1 - 2008
N2 - The herb Phyllanthus reticulatus is commonly known as potato bush, which is widely used in the indigenous system of medicine for the treatment of diarrhoea, diabetes, obesity and burns. The methanolic extract of the plant was studied for its in vitro free radical scavenging activity in different methods viz DPPH radical scavenging assay, ABTS radical scavenging assay, Ophenanthroline assay, lipid peroxidation assay, nitric oxide scavenging assay, superoxide scavenging assay, total antioxidant and non-enzymatic haemoglobin glycosylation assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC 50 value and the values were 14.31 μgm/ml (DPPH radical scavenging), 10.51 μgm/ml (ABTS radical scavenging), 22.91 μgm/ml (O-phenanthroline assay), 32.16 μgm/ml (lipid peroxidation), 90.51 μgm/ml (nitric oxide scavenging) and 4.18 μgm/ml (superoxide scavenging). In total antioxidant capacity assay, 1 mg of extract is equivalent to 41.31 μg of ascorbic acid. It showed 83.69% inhibition of haemoglobin glycosylation.
AB - The herb Phyllanthus reticulatus is commonly known as potato bush, which is widely used in the indigenous system of medicine for the treatment of diarrhoea, diabetes, obesity and burns. The methanolic extract of the plant was studied for its in vitro free radical scavenging activity in different methods viz DPPH radical scavenging assay, ABTS radical scavenging assay, Ophenanthroline assay, lipid peroxidation assay, nitric oxide scavenging assay, superoxide scavenging assay, total antioxidant and non-enzymatic haemoglobin glycosylation assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC 50 value and the values were 14.31 μgm/ml (DPPH radical scavenging), 10.51 μgm/ml (ABTS radical scavenging), 22.91 μgm/ml (O-phenanthroline assay), 32.16 μgm/ml (lipid peroxidation), 90.51 μgm/ml (nitric oxide scavenging) and 4.18 μgm/ml (superoxide scavenging). In total antioxidant capacity assay, 1 mg of extract is equivalent to 41.31 μg of ascorbic acid. It showed 83.69% inhibition of haemoglobin glycosylation.
M3 - Article
SN - 1827-8620
VL - 2
SP - 440
EP - 451
JO - Pharmacologyonline
JF - Pharmacologyonline
ER -
