Influence of Buffers on Intrinsically Disordered Proteins Using Autofluorescence Spectroscopy

Anjana Pithakumar; Shaik Basha; Shanmukha Sreeya Devarakonda; Thoshna; Darshan Chikkanayakanahalli Mukunda; Jackson Rodrigues; Subhash Chandra; K. Ameera; Shimul Biswas; Krishna Kishore Mahato

Influence of Buffers on Intrinsically Disordered Proteins Using Autofluorescence Spectroscopy

Anjana Pithakumar
, Shaik Basha
, Shanmukha Sreeya Devarakonda
, Thoshna
, Darshan Chikkanayakanahalli Mukunda
, Jackson Rodrigues
, Subhash Chandra
, K. Ameera
, Shimul Biswas
, Krishna Kishore Mahato^*

^*Corresponding author for this work

Department of Biophysics, Manipal School of Life Sciences, Manipal

Research output: Contribution to conference › Paper › peer-review

Abstract

The current study employs Light Emitting Diode-Induced Autofluorescence (LED-IAF) spectroscopy to assess the influence of different pH buffers on intrinsically disordered proteins. Further, the action of buffers was validated by Thioflavin T assay.

Original language	English
Publication status	Published - 2024
Event	2024 Laser Science, LS 2024 - Denver, United States Duration: 23-09-2024 → 26-09-2024

Conference

Conference	2024 Laser Science, LS 2024
Country/Territory	United States
City	Denver
Period	23-09-24 → 26-09-24

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering
Electronic, Optical and Magnetic Materials

Cite this

@conference{8f638559f0b74fb6bae7ed4f64d877f2,

title = "Influence of Buffers on Intrinsically Disordered Proteins Using Autofluorescence Spectroscopy",

abstract = "The current study employs Light Emitting Diode-Induced Autofluorescence (LED-IAF) spectroscopy to assess the influence of different pH buffers on intrinsically disordered proteins. Further, the action of buffers was validated by Thioflavin T assay.",

author = "Anjana Pithakumar and Shaik Basha and Devarakonda, \{Shanmukha Sreeya\} and Thoshna and Mukunda, \{Darshan Chikkanayakanahalli\} and Jackson Rodrigues and Subhash Chandra and K. Ameera and Shimul Biswas and Mahato, \{Krishna Kishore\}",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s). {\textcopyright} Optica Publishing Group 2024.; 2024 Laser Science, LS 2024 ; Conference date: 23-09-2024 Through 26-09-2024",

year = "2024",

language = "English",

}

TY - CONF

T1 - Influence of Buffers on Intrinsically Disordered Proteins Using Autofluorescence Spectroscopy

AU - Pithakumar, Anjana

AU - Basha, Shaik

AU - Devarakonda, Shanmukha Sreeya

AU - Thoshna,

AU - Mukunda, Darshan Chikkanayakanahalli

AU - Rodrigues, Jackson

AU - Chandra, Subhash

AU - Ameera, K.

AU - Biswas, Shimul

AU - Mahato, Krishna Kishore

PY - 2024

Y1 - 2024

N2 - The current study employs Light Emitting Diode-Induced Autofluorescence (LED-IAF) spectroscopy to assess the influence of different pH buffers on intrinsically disordered proteins. Further, the action of buffers was validated by Thioflavin T assay.

AB - The current study employs Light Emitting Diode-Induced Autofluorescence (LED-IAF) spectroscopy to assess the influence of different pH buffers on intrinsically disordered proteins. Further, the action of buffers was validated by Thioflavin T assay.

UR - https://www.scopus.com/pages/publications/85215940242

UR - https://www.scopus.com/pages/publications/85215940242#tab=citedBy

M3 - Paper

AN - SCOPUS:85215940242

T2 - 2024 Laser Science, LS 2024

Y2 - 23 September 2024 through 26 September 2024

ER -

Influence of Buffers on Intrinsically Disordered Proteins Using Autofluorescence Spectroscopy

Abstract

Conference

All Science Journal Classification (ASJC) codes

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